PE Anti-Ikaros antibody [4E9] (ab232924)

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Flow Cytometry - PE Anti-Ikaros antibody [4E9] (ab232924)

    Key features and details

    • PE Mouse monoclonal [4E9] to Ikaros
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-Ikaros antibody [4E9]
      See all Ikaros primary antibodies

    • Description

      PE Mouse monoclonal [4E9] to Ikaros

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse, Human

    • Immunogen

      Recombinant fragment corresponding to Human Ikaros (C terminal).

    • Positive control

      • Flow Cytometry: Human peripheral blood leukocytes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab232924 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    Target

    • Function

      Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).

    • Tissue specificity

      Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

    • Involvement in disease

      Defects in IKZF1 are frequent occurrences (28.6%) in acute lymphoblasic leukemia (ALL). Such alterations or deletions lead to poor prognosis for ALL.
      Chromosomal aberrations involving IKZF1 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;7)(q27;p12), with BCL6.

    • Sequence similarities

      Belongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.

    • Domain

      The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin.
      The C-terminal zinc-finger domain is required for dimerization.

    • Post-translational
      modifications

      Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
      Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1.
      Polyubiquitinated.

    • Cellular localization

      Cytoplasm; Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events and Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity).
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 7 isoforms produced by alternative splicing.

    • Alternative names

      • CLL associated antigen KW 6 antibody
      • DNA-binding protein Ikaros antibody
      • hIk 1 antibody
      • Hs.54452 antibody
      • IK1 antibody
      • Ikaros (zinc finger protein) antibody
      • IKAROS antibody
      • IKAROS family zinc finger 1 (Ikaros) antibody
      • Ikaros family zinc finger protein 1 antibody
      • Ikzf1 antibody
      • IKZF1_HUMAN antibody
      • LYF1 antibody
      • Lymphoid transcription factor LyF-1 antibody
      • PRO0758 antibody
      • Zinc finger protein subfamily 1A 1 (Ikaros) antibody
      • Zinc finger protein subfamily 1A 1 antibody
      • Zinc finger protein, subfamily 1A, member 1 antibody
      • ZNFN1A1 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-Ikaros antibody [4E9] (ab232924)
      Flow Cytometry - PE Anti-Ikaros antibody [4E9] (ab232924)

      Intracellular staining of Ikaros in human peripheral blood leukocytes with ab232924. Gated on leukocytes.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232924? Please let us know so that we can cite the reference in this datasheet.

    ab232924 has not yet been referenced specifically in any publications.

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