Key features and details
- PE Rat monoclonal [13A] to IL-13
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-IL-13 antibody [13A]
See all IL-13 primary antibodies
DescriptionPE Rat monoclonal [13A] to IL-13
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant full length protein corresponding to Mouse IL-13.
- BALB/c splenocytes
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab95576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25µg for 105-8 cells.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
Involvement in diseaseAllergic rhinitis
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
ab95576 at 0.125 µg/test staining IL-13 by Flow Cytometry.
BALB/c splenocytes were cultured for 3 days with plate-bound Anti-Mouse CD3e, soluble Anti-Mouse CD28, Mouse IL2 Recombinant Protein and Mouse IL4 Recombinant Protein. Cells were then harvested and restimulated with PMA and Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with Anti-Mouse CD4 FITC, fixed and permeabilized, then stained with 0.125 µg of ab95576 (1). The ligand blocking control demonstrates that pre-blocking ab95576 with 1.0 µg of Mouse IL-13 Recombinant Protein prior to staining abrogates specific mouse IL-13 staining (2).
ab95576 has been referenced in 1 publication.
- Poe C et al. Treatment of established TH2 cells with 4µ8c, an inhibitor of IRE1a, blocks IL-5 but not IL-4 secretion. BMC Immunol 20:3 (2019). PubMed: 30630412