PE Anti-IL-2 Receptor alpha antibody [7D4] (ab24933)

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Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [7D4] (ab24933)

    Key features and details

    • PE Rat monoclonal [7D4] to IL-2 Receptor alpha
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgM

    Overview

    • Product name

      PE Anti-IL-2 Receptor alpha antibody [7D4]
      See all IL-2 Receptor alpha primary antibodies

    • Description

      PE Rat monoclonal [7D4] to IL-2 Receptor alpha

    • Host species

      Rat

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      This antibody recognises low affinity IL-2 receptor alpha.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue, cells or virus corresponding to Mouse IL-2 Receptor alpha.

    • Epitope

      This antibody reacts with an epitope located between amino acids 125 and 212 of IL-2 receptor alpha.

    • Positive control

      • Cells from BALB/c spleen activated with PMA and ionomycin for Immunofluorescent staining with Flow Cytometric analysis.

    • General notes

       

      This antibody does not block the binding of IL-2 to CD25.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab24933 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.2µg for 106 cells.

    ab154451 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Receptor for interleukin-2.

    • Involvement in disease

      Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

    • Sequence similarities

      Contains 2 Sushi (CCP/SCR) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Interleukin 2 receptor alpha chain antibody
        • CD25 antibody
        • CD25 antigen antibody
        • IDDM10 antibody
        • IL 2 receptor alpha subunit antibody
        • IL-2 receptor subunit alpha antibody
        • IL-2-RA antibody
        • IL-2R subunit alpha antibody
        • IL2 RA antibody
        • IL2 Receptor alpha antibody
        • IL2-RA antibody
        • IL2R antibody
        • IL2R, alpha chain antibody
        • IL2RA antibody
        • IL2RA_HUMAN antibody
        • IMD41 antibody
        • Interleukin 2 receptor alpha antibody
        • Interleukin 2 receptor antibody
        • Interleukin-2 receptor subunit alpha antibody
        • p55 antibody
        • t-cell growth factor receptor antibody
        • TAC antibody
        • TAC antigen antibody
        • TCGFR antibody
        see all

      Images

      • Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [7D4] (ab24933)
        Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [7D4] (ab24933)
        Ab24933, at a concentration of 0.1µg for 106 cells, staining CD25 in cells from BALB/c spleen that had been activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours. Stained cells were then analyzed on a flow cytometer.

      References (0)

      Publishing research using ab24933? Please let us know so that we can cite the reference in this datasheet.

      ab24933 has not yet been referenced specifically in any publications.

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