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    pe-il-2-receptor-alpha-antibody-bc96-ab134476.pdf

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Immunology Adaptive Immunity T Cells CD
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PE Anti-IL-2 Receptor alpha antibody [BC96] (ab134476)

  • Datasheet
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Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [BC96] (ab134476)

    Key features and details

    • PE Mouse monoclonal [BC96] to IL-2 Receptor alpha
    • Suitable for: Flow Cyt
    • Reacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)

    View more associated products

    Overview

    • Product name

      PE Anti-IL-2 Receptor alpha antibody [BC96]
      See all IL-2 Receptor alpha primary antibodies
    • Description

      PE Mouse monoclonal [BC96] to IL-2 Receptor alpha
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • PHA-stimulated normal Human peripheral blood cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      BC96
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Cytokines
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Alternative Versions

      • PE/Cy7® Anti-IL-2 Receptor alpha antibody [BC96] (ab134479)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
    • Recombinant Protein

      • Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)

    Applications

    Our Abpromise guarantee covers the use of ab134476 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Target

    • Function

      Receptor for interleukin-2.
    • Involvement in disease

      Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    • Sequence similarities

      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P01589 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 450884 Chimpanzee
      • Entrez Gene: 3559 Human
      • Entrez Gene: 574300 Rhesus monkey
      • Omim: 147730 Human
      • SwissProt: Q38IC8 Chimpanzee
      • SwissProt: P01589 Human
      • SwissProt: Q5MNY4 Rhesus monkey
      • Unigene: 231367 Human
      • Alternative names

        • Interleukin 2 receptor alpha chain antibody
        • CD25 antibody
        • CD25 antigen antibody
        • IDDM10 antibody
        • IL 2 receptor alpha subunit antibody
        • IL-2 receptor subunit alpha antibody
        • IL-2-RA antibody
        • IL-2R subunit alpha antibody
        • IL2 RA antibody
        • IL2 Receptor alpha antibody
        • IL2-RA antibody
        • IL2R antibody
        • IL2R, alpha chain antibody
        • IL2RA antibody
        • IL2RA_HUMAN antibody
        • IMD41 antibody
        • Interleukin 2 receptor alpha antibody
        • Interleukin 2 receptor antibody
        • Interleukin-2 receptor subunit alpha antibody
        • p55 antibody
        • t-cell growth factor receptor antibody
        • TAC antibody
        • TAC antigen antibody
        • TCGFR antibody
        see all

      Images

      • Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [BC96] (ab134476)
        Flow Cytometry - PE Anti-IL-2 Receptor alpha antibody [BC96] (ab134476)

        Flow Cytometry analysis of human peripheral blood lymphocytes labeling IL-2 Receptor alpha with ab134476 at 5 µL (0.06 µg) (right panel) or 0.06 µg Mouse IgG1 (Phycoerythrin)  isotype control (left panel).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab134476? Please let us know so that we can cite the reference in this datasheet.

      ab134476 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-IL-2 Receptor alpha antibody [BC96] (Phycoerythrin)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (PBMC)
      Permeabilization
      No
      Gating Strategy
      lymphocytes
      Specification
      PBMC
      Preparation
      Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
      Sample buffer: PBS
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 19 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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