Key features and details
- PE Rat monoclonal [PC61.5] to IL-2 Receptor alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-IL-2 Receptor alpha antibody [PC61.5]
See all IL-2 Receptor alpha primary antibodies
DescriptionPE Rat monoclonal [PC61.5] to IL-2 Receptor alpha
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- C57Bl/6 mouse spleen cells
ab210334 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25-.
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
Concentration information loading...
Purification notesab210334 was purified from tissue culture supernatant.
Light chain typelambda
- APC Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210330)
- FITC Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210332)
- Anti-IL-2 Receptor alpha antibody [PC61.5] - Low endotoxin, Azide free (ab210333)
- PE/Cy7® Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210335)
- PerCP/Cy5.5® Anti-IL-2 Receptor alpha antibody [PC61.5] (ab210336)
Our Abpromise guarantee covers the use of ab210334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Use at 0.125 µg.
FunctionReceptor for interleukin-2.
Involvement in diseaseGenetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesContains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
ab210334 has not yet been referenced specifically in any publications.