PE Anti-IL2 Receptor beta/p75 antibody [5H4] (ab95585)
![Flow Cytometry - PE Anti-IL2 Receptor beta/p75 antibody [5H4] (ab95585)](https://www.abcam.com/ps/products/95/ab95585/Images/ab95585-109862-anti-il2-receptor-beta-antibody-5h4-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-IL2 Receptor beta/p75 antibody [5H4] (ab95585)")
Key features and details
- PE Rat monoclonal [5H4] to IL2 Receptor beta/p75
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-IL2 Receptor beta/p75 antibody [5H4]
See all IL2 Receptor beta/p75 primary antibodies -
Description
PE Rat monoclonal [5H4] to IL2 Receptor beta/p75 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse IL2 Receptor beta/p75. Mouse IL2 Receptor beta/p75 transfected rat cell line.
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Positive control
- BALB/c splenocytes
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General notes
Binding of IL2 to IL2 receptor beta is not inhibited by the ab95585.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Binding of IL2 to IL2 receptor beta is not inhibited by the ab95585. -
Clonality
Monoclonal -
Clone number
5H4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.125µg for 105-8 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 0.125µg for 105-8 cells. |
Target
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Function
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16185 Mouse
- SwissProt: P16297 Mouse
- Unigene: 35287 Mouse
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Alternative names
- CD122 antibody
- CD122 antigen antibody
- High affinity IL 2 receptor beta subunit antibody
see all
Images
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Flow Cytometry - PE Anti-IL2 Receptor beta/p75 antibody [5H4] (ab95585)Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of ab95585 (filled histogram). Total viable cells were used for analysis.
Datasheets and documents
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Datasheet download
References (0)
ab95585 has not yet been referenced specifically in any publications.
