PE Anti-ILT-4 antibody [42D1] (ab95820)
![Flow Cytometry - PE Anti-ILT-4 antibody [42D1] (ab95820)](https://www.abcam.com/ps/products/95/ab95820/Images/ab95820-110066-anti-ilt-4-antibody-42d1-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-ILT-4 antibody [42D1] (ab95820)")
Key features and details
- PE Rat monoclonal [42D1] to ILT-4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-ILT-4 antibody [42D1]
See all ILT-4 primary antibodies -
Description
PE Rat monoclonal [42D1] to ILT-4 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ILT-4.
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Positive control
- Normal human peripheral blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
42D1 -
Isotype
IgG2a -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10288 Human
- Omim: 604815 Human
- SwissProt: Q8N423 Human
- Unigene: 655652 Human
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Alternative names
- CD85 antigen-like family member D antibody
- CD85d antibody
- CD85d antigen antibody
see all
Images
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Flow Cytometry - PE Anti-ILT-4 antibody [42D1] (ab95820)
ab95820 staining ILT-4 in normal Human peripheral blood cells by Flow Cytometry. Cells are co-stained with anti-Human CD14 APC. Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.
Datasheets and documents
References (0)
ab95820 has not yet been referenced specifically in any publications.
