PE Anti-Integrin alpha 2 antibody [DX5] (ab25493)
Key features and details
- PE Rat monoclonal [DX5] to Integrin alpha 2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgM
Overview
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Product name
PE Anti-Integrin alpha 2 antibody [DX5]
See all Integrin alpha 2 primary antibodies -
Description
PE Rat monoclonal [DX5] to Integrin alpha 2 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab25493 recognises integrin alpha 2. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue/ cell preparation: RIL 2 propagated NK1.1 positive cells from C57BL/6 mice.
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General notes
NK cells cultured in the presence of IL2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. DX5 has not been demonstrated to have activating or blocking activity.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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Purity
Purified IgM -
Primary antibody notes
NK cells cultured in the presence of IL2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. DX5 has not been demonstrated to have activating or blocking activity. -
Clonality
Monoclonal -
Clone number
DX5 -
Myeloma
Sp2/0 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | Use 0.2µg for 106 cells. ab25493 can be used to define functionally distinct subsets of murine NK cells. |
Target
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Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16398 Mouse
- SwissProt: Q62469 Mouse
- Unigene: 5007 Mouse
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Alternative names
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
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Images
Datasheets and documents
References (1)
ab25493 has been referenced in 1 publication.
- Arase H et al. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol 167:1141-4 (2001). PubMed: 11466327