Recombinant PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
![Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)](https://www.abcam.com/ps/products/225/ab225284/Images/ab225284-333782-anti-integrin-alpha-2-antibody-epr17338-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR17338] to Integrin alpha 2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Integrin alpha 2 antibody [EPR17338]
See all Integrin alpha 2 primary antibodies -
Description
PE Rabbit monoclonal [EPR17338] to Integrin alpha 2 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17338 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
- Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab208770)
- Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209766)
- HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
- Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab210655)
- Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
- Anti-Integrin alpha 2 antibody [EPR17338] - BSA and Azide free (ab271936)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3673 Human
- Entrez Gene: 16398 Mouse
- Entrez Gene: 170921 Rat
- Omim: 192974 Human
- SwissProt: P17301 Human
- SwissProt: Q62469 Mouse
- Unigene: 482077 Human
- Unigene: 5007 Mouse
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Alternative names
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
Overlay histogram showing A431 cells stained with ab225284 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225284, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)](https://www.abcam.com/ps/products/225/ab225284/Images/ab225284-3-benefits-of-recombinant-antibodies.png)
PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab225284 has not yet been referenced specifically in any publications.
