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    pe-integrin-alpha-2-antibody-epr17338-ab225284.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
  • PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR17338] to Integrin alpha 2
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free HRP Unconjugated Unconjugated

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Recombinant Human Integrin alpha 2 protein (ab158772)
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Overview

  • Product name

    PE Anti-Integrin alpha 2 antibody [EPR17338]
    See all Integrin alpha 2 primary antibodies
  • Description

    PE Rabbit monoclonal [EPR17338] to Integrin alpha 2
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): A431 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17338
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
    • Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab208770)
    • Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209766)
    • HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
    • Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab210655)
    • Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
    • Anti-Integrin alpha 2 antibody [EPR17338] - BSA and Azide free (ab271936)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant Human Integrin alpha 2 protein (ab158772)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Notes
Flow Cyt (Intra)
1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P17301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3673 Human
    • Entrez Gene: 16398 Mouse
    • Entrez Gene: 170921 Rat
    • Omim: 192974 Human
    • SwissProt: P17301 Human
    • SwissProt: Q62469 Mouse
    • Unigene: 482077 Human
    • Unigene: 5007 Mouse
    • Alternative names

      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha II antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
      Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)

      Overlay histogram showing A431 cells stained with ab225284 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225284, 1/1000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    • PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
      PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab225284? Please let us know so that we can cite the reference in this datasheet.

    ab225284 has not yet been referenced specifically in any publications.

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