PE Anti-Integrin alpha 4/CD49D antibody [9F10] (ab269294)
![Flow Cytometry - PE Anti-Integrin alpha 4/CD49D antibody [9F10] (ab269294)](https://www.abcam.com/ps/products/269/ab269294/Images/ab269294-1-AntiCD49dPEantibodySheepflow-cytometry.jpg "Flow Cytometry - PE Anti-Integrin alpha 4/CD49D antibody [9F10] (ab269294)")
Key features and details
- PE Mouse monoclonal [9F10] to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-Integrin alpha 4/CD49D antibody [9F10]
See all Integrin alpha 4/CD49D primary antibodies -
Description
PE Mouse monoclonal [9F10] to Integrin alpha 4/CD49D -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab269294 recognizes an extracellular epitope of CD49d.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Horse, Cow, Cat, Dog, Non human primates
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow cyt: Human peripheral blood leukocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
9F10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab269294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. |
Target
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Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
Post-translational
modificationsPhosphorylation on Ser-1027 inhibits PXN binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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Alternative names
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
Images
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Flow Cytometry - PE Anti-Integrin alpha 4/CD49D antibody [9F10] (ab269294)Flow cytometry analysis (surface staining) of CD49d in human peripheral blood leukocytes labeling CD49d with ab269294.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab269294 has not yet been referenced specifically in any publications.
