Recombinant PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
![Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)](https://www.abcam.com/ps/products/211/ab211845/Images/ab211845-264933-anti-integrin-alpha-v-antibody-epr16800-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR16800] to Integrin alpha V
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Integrin alpha V antibody [EPR16800]
See all Integrin alpha V primary antibodies -
Description
PE Rabbit monoclonal [EPR16800] to Integrin alpha V -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
Database link: P06756 -
Positive control
- Flow Cyt: A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR16800 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Integrin alpha V antibody [EPR16800] (ab179475)
- Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800] (ab204684)
- HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
- Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800] (ab207286)
- Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
- Alexa Fluor® 488 Anti-Integrin alpha V antibody [EPR16800] (ab275350)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab211845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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Flow Cytometry - PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)Overlay histogram showing A549 cells stained with ab211845 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211845, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. -
![PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)](https://www.abcam.com/ps/products/211/ab211845/Images/ab211845-3-benefits-of-recombinant-antibodies.png)
PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
References (0)
ab211845 has not yet been referenced specifically in any publications.
