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PE Anti-Integrin beta 1 antibody [HM beta 1-1] (ab36219)

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Flow Cytometry - PE Anti-Integrin beta 1 antibody [HM beta 1-1] (ab36219)

    Key features and details

    • PE Armenian hamster monoclonal [HM beta 1-1] to Integrin beta 1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse, Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG

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    Overview

    • Product name

      PE Anti-Integrin beta 1 antibody [HM beta 1-1]
      See all Integrin beta 1 primary antibodies
    • Description

      PE Armenian hamster monoclonal [HM beta 1-1] to Integrin beta 1
    • Host species

      Armenian hamster
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse, Human
    • Immunogen

      Purified VLA4 antigen (Mouse).

    • General notes


      This clone is reported to inhibit beta 1 integrin mediated adhesion.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from tissue culture supernatant.
    • Primary antibody notes

      This clone is reported to inhibit beta 1 integrin mediated adhesion.
    • Clonality

      Monoclonal
    • Clone number

      HM beta 1-1
    • Myeloma

      P3U1
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neural Stem Cell marker
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Stem Cells
      • Embryonic Stem Cells
      • Surface Molecules
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Developmental Biology
      • Embryogenesis
      • Embryonic stem cells
      • Intracellular

    Associated products

    • Isotype control

      • PE Armenian Hamster IgG monoclonal - Isotype control (ab101026)
    • Recombinant Protein

      • Recombinant Human Integrin beta 1 protein (ab114157)

    Applications

    Our Abpromise guarantee covers the use of ab36219 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/5.

    Use 10 µl of the suggested working dilution to label 106 cells in 100 µl.

     

     

     

    ab101026 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
      Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
      (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
    • Tissue specificity

      Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications

      The cysteine residues are involved in intrachain disulfide bonds.
    • Cellular localization

      Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
    • Target information above from: UniProt accession P05556 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3688 Human
      • Entrez Gene: 16412 Mouse
      • Omim: 135630 Human
      • SwissProt: P05556 Human
      • SwissProt: P09055 Mouse
      • Unigene: 643813 Human
      • Unigene: 263396 Mouse
      • Alternative names

        • beta1 integrin antibody
        • CD_antigen=CD29 antibody
        • CD29 antibody
        • Fibronectin receptor subunit beta antibody
        • FNRB antibody
        • Glycoprotein IIa antibody
        • GP IIa antibody
        • GPIIA antibody
        • Integrin beta-1 antibody
        • Integrin subunit beta 1 antibody
        • integrin VLA-4 beta subunit antibody
        • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
        • ITB1_HUMAN antibody
        • ITGB1 antibody
        • MDF2 antibody
        • MSK12 antibody
        • OTTHUMP00000019420 antibody
        • very late activation protein antibody
        • Very late activation protein, beta polypeptide antibody
        • VLA BETA antibody
        • VLA-4 subunit beta antibody
        • VLA-BETA antibody
        • VLAB antibody
        • VLAbeta antibody
        see all

      Images

      • Flow Cytometry - PE Anti-Integrin beta 1 antibody [HM beta 1-1] (ab36219)
        Flow Cytometry - PE Anti-Integrin beta 1 antibody [HM beta 1-1] (ab36219)
        ab36219 staining Integrin beta 1 in mouse thymocytes by Flow Cytometric analysis.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (5)

      Publishing research using ab36219? Please let us know so that we can cite the reference in this datasheet.

      ab36219 has been referenced in 5 publications.

      • Legan SK  et al. a5ß1 integrin mediates pulmonary epithelial cyst formation. Dev Dyn 246:475-484 (2017). PubMed: 28370666
      • Ghazvini M  et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS One 8:e73872 (2013). PubMed: 24069241
      • Maerckx C  et al. Hepato-biliary profile of potential candidate liver progenitor cells from healthy rat liver. World J Gastroenterol 18:3511-9 (2012). Flow Cyt ; Rat . PubMed: 22826615
      • Fierro FA  et al. BCR/ABL expression of myeloid progenitors increases beta1-integrin mediated adhesion to stromal cells. J Mol Biol 377:1082-93 (2008). Flow Cyt ; Human . PubMed: 18313694
      • Noto K  et al. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol 7:835-42 (1995). PubMed: 7547709

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