PE Anti-Interferon gamma antibody [CC302] (ab27866)

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Key features and details

  • PE Mouse monoclonal [CC302] to Interferon gamma
  • Suitable for: Flow Cyt
  • Reacts with: Sheep, Goat, Horse, Cow, Dog, Human, Pig, Ferret
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-Interferon gamma antibody [CC302]
    See all Interferon gamma primary antibodies

  • Description

    PE Mouse monoclonal [CC302] to Interferon gamma

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody recognises bovine interferon gamma

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Sheep, Goat, Horse, Cow, Dog, Human, Pig, Ferret

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
1/1 - 1/10.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.
Notes
Flow Cyt
1/1 - 1/10.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • Tissue specificity

    Released primarily from activated T lymphocytes.

  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.

  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • IF 1 antibody
    • IFG antibody
    • IFI antibody
    • ifn g antibody
    • IFN gamma antibody
    • IFN immune antibody
    • IFN, immune antibody
    • IFN-gamma antibody
    • IFNG antibody
    • IFNG_HUMAN antibody
    • Immune interferon antibody
    • Interferon gamma antibody
    • Type II Interferon antibody
    see all

References (0)

Publishing research using ab27866? Please let us know so that we can cite the reference in this datasheet.

ab27866 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Flow Cytometry abreview for Anti-Interferon gamma antibody [CC302] (PE)

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Dog Cell (T cell)
Permeabilization
Yes - kit
Gating Strategy
CD5+
Specification
T cell
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Verified customer

Submitted Aug 19 2020

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