PE Anti-LAIR1 antibody [113] (ab95749)
![Flow Cytometry - PE Anti-LAIR1 antibody [113] (ab95749)](https://www.abcam.com/ps/products/95/ab95749/Images/ab95749-109995-anti-lair1-antibody-113-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-LAIR1 antibody [113] (ab95749)")
Key features and details
- PE Armenian hamster monoclonal [113] to LAIR1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-LAIR1 antibody [113]
See all LAIR1 primary antibodies -
Description
PE Armenian hamster monoclonal [113] to LAIR1 -
Host species
Armenian hamster -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
ARHO12 clone stably expressing Mouse LAIR1 fused to a C terminal MYC tag.
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Positive control
- C57BL/6 splenocytes
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.125-0.25µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample. |
Target
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Function
Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. -
Tissue specificity
Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation. -
Domain
ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation. -
Post-translational
modificationsPhosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
N-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 52855 Mouse
- SwissProt: Q8BG84 Mouse
- Unigene: 290880 Mouse
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Alternative names
- 5133400O11Rik antibody
- CD305 antibody
- D7Bwg0421e antibody
see all
Images
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Flow Cytometry - PE Anti-LAIR1 antibody [113] (ab95749)FLow cytometry staining of C57BL/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control PE (blue histogram) or 0.125 µg ab95749 (purple histogram). Total viable F4/80+ cells were used for analysis.
Datasheets and documents
References (1)
ab95749 has been referenced in 1 publication.
- Lebbink RJ et al. Mouse leukocyte-associated Ig-like receptor-1 (mLAIR-1) functions as an inhibitory collagen-binding receptor on immune cells. Int Immunol 19:1011-9 (2007). Flow Cyt . PubMed: 17702987
