Recombinant PE Anti-LDL Receptor antibody [032] (ab275705)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [032] to LDL Receptor
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-LDL Receptor antibody [032]
See all LDL Receptor primary antibodies -
Description
PE Rabbit monoclonal [032] to LDL Receptor -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse LDL Receptor aa 1-790 (extracellular). With substitution of Val 23 and Gly 27 by Ala 23 and Cys 27 respectively.
Database link: P35951 -
Positive control
- Flow Cyt: RAW 264.7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
032 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
5 µl/Test. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 5 µl/Test. |
Target
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Function
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
Involvement in disease
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
Cellular localization
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
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Database links
- Entrez Gene: 16835 Mouse
- SwissProt: P35951 Mouse
- Unigene: 3213 Mouse
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Alternative names
- FH antibody
- FHC antibody
- LDL R antibody
see all
Images
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Profile of anti-LDLR reactivity on RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with a mouse BD Fc Block purified anti-CD16/CD32 mAb 2.4G2 prior to staining, washed, then stained with ab275705 at 5 µl/Test.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275705 has not yet been referenced specifically in any publications.