PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)
![Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)](https://www.abcam.com/ps/products/186/ab186074/Images/ab186074-1-anti-cd85j-LILRB1-antibody-ghi75-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)")
Key features and details
- PE Mouse monoclonal [GHI/75] to LILRB1, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-LILRB1 antibody [GHI/75], prediluted
See all LILRB1 primary antibodies -
Description
PE Mouse monoclonal [GHI/75] to LILRB1, prediluted -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human LILRB1. Hairy cell leukaemia cells.
Database link: Q8NHL6 -
Positive control
- Flow Cyt: Human blood cells.
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General notes
ab186074 recognizes LILRB1, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
GHI/75 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab186074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
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Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
Tissue specificity
Expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells. Detected on a low percentage of T-cells and natural killer (NK) cells. -
Sequence similarities
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10859 Human
- Omim: 604811 Human
- SwissProt: Q8NHL6 Human
- Unigene: 667388 Human
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Alternative names
- CD85 antibody
- CD85 antigen antibody
- CD85 antigen like family member J antibody
see all
Images
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Flow Cytometry - PE Anti-LILRB1 antibody [GHI/75], prediluted (ab186074)Flow cytometry analysis of human peripheral blood labeling CD85j/LILRB1 using ab186074 at 10 µL/100 µL whole blood. Surface staining.
Datasheets and documents
References (0)
ab186074 has not yet been referenced specifically in any publications.
