PE Anti-LIME antibody [LIME-06] (ab64678)
Key features and details
- PE Mouse monoclonal [LIME-06] to LIME
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-LIME antibody [LIME-06]
See all LIME primary antibodies -
Description
PE Mouse monoclonal [LIME-06] to LIME -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to aa 141-295 of human LIME.
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Positive control
- Peripheral blood T cells stimulated with anti-human CD4.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody (>95% by SDS-PAGE) was conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
LIME-06 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Target
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Relevance
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. -
Cellular localization
Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell -
Database links
- Entrez Gene: 54923 Human
- Omim: 609809 Human
- SwissProt: Q9H400 Human
- Unigene: 233220 Human
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Alternative names
- dJ583P15.4 antibody
- FLJ20406 antibody
- Lck interacting membrane protein antibody
see all
References (0)
ab64678 has not yet been referenced specifically in any publications.
