Recombinant PE Anti-LRP1 antibody [EPR3724] (ab225060)
![Flow Cytometry - PE Anti-LRP1 antibody [EPR3724] (ab225060)](https://www.abcam.com/ps/products/225/ab225060/Images/ab225060-292254-anti-lrp1-antibody-epr3724-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-LRP1 antibody [EPR3724] (ab225060)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR3724] to LRP1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-LRP1 antibody [EPR3724]
See all LRP1 primary antibodies -
Description
PE Rabbit monoclonal [EPR3724] to LRP1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human, Pig
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Immunogen
Synthetic peptide within Human LRP1 aa 4450 to the C-terminus. The exact sequence is proprietary.
Database link: Q07954 -
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR3724 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-LRP1 antibody [EPR3724] (ab195567)
- Alexa Fluor® 647 Anti-LRP1 antibody [EPR3724] (ab195568)
- HRP Anti-LRP1 antibody [EPR3724] (ab195569)
- Alexa Fluor® 594 Anti-LRP1 antibody [EPR3724] (ab206902)
- Alexa Fluor® 555 Anti-LRP1 antibody [EPR3724] (ab206904)
- Anti-LRP1 antibody [EPR3724] - BSA and Azide free (ab215997)
- Anti-LRP1 antibody [EPR3724] (ab92544)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab225060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
Tissue specificity
Most abundant in liver, brain and lung. -
Sequence similarities
Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
Post-translational
modificationsCleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
Cellular localization
Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit. - Information by UniProt
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Database links
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Entrez Gene: 299858 Rat
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
- Unigene: 271854 Mouse
see all -
Alternative names
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
Images
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Flow Cytometry - PE Anti-LRP1 antibody [EPR3724] (ab225060)
Overlay histogram showing Neuro-2a cells stained with ab225060 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225060, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in Neuro-2a cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![PE Anti-LRP1 antibody [EPR3724] (ab225060)](https://www.abcam.com/ps/products/225/ab225060/Images/ab225060-3-benefits-of-recombinant-antibodies.png)
PE Anti-LRP1 antibody [EPR3724] (ab225060)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225060 has not yet been referenced specifically in any publications.
