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  1. Link

    pe-ly6c-antibody-hk14-ab25572.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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PE Anti-Ly6c antibody [HK1.4] (ab25572)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Abpromise

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Key features and details

  • PE Rat monoclonal [HK1.4] to Ly6c
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2c

You may also be interested in

Protein
Product image
Recombinant Mouse Ly6c protein (Tagged) (ab240852)

View more associated products

Overview

  • Product name

    PE Anti-Ly6c antibody [HK1.4]
    See all Ly6c primary antibodies
  • Description

    PE Rat monoclonal [HK1.4] to Ly6c
  • Host species

    Rat
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Specificity

    ab25572 recognises Ly6c.
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 16% Sucrose

    Also contains a stabilizing agent.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    HK1.4
  • Isotype

    IgG2c
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Anti-Ly6c antibody [HK1.4] (ab24973)
    • Biotin Anti-Ly6c antibody [HK1.4] (ab24980)
    • FITC Anti-Ly6c antibody [HK1.4] (ab25025)
    • APC Anti-Ly6c antibody [HK1.4] (ab93550)
  • Isotype control

    • PE Rat IgG2c, kappa monoclonal [SB68b] - Isotype control (ab154450)
  • Recombinant Protein

    • Recombinant Mouse Ly6c protein (Tagged) (ab240852)

Target

  • Relevance

    Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localization

    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links

    • Entrez Gene: 17067 Mouse
    • SwissProt: P0CW02 Mouse
    • SwissProt: P0CW03 Mouse
    • Alternative names

      • Ly 6c antibody
      • Ly6c protein antibody
      • Ly6c1 antibody
      • Ly6c2 antibody
      • Lymphocyte antigen 6 complex antibody
      • Lymphocyte antigen 6 complex locus C antibody
      • Lymphocyte antigen 6C antibody
      • Lymphocyte antigen 6C1 antibody
      • Lymphocyte antigen 6C2 antibody
      • Lymphocyte antigen Ly 6C antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab25572? Please let us know so that we can cite the reference in this datasheet.

    ab25572 has been referenced in 2 publications.

    • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234
    • Ocuin LM  et al. Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. J Leukoc Biol 89:423-32 (2011). Flow Cyt ; Mouse . PubMed: 21106642

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Anti-Ly6c antibody [HK1.4] (Phycoerythrin)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (splenocytes)
    Specification
    splenocytes
    Preparation
    Cell harvesting/tissue preparation method: mechanical disruption of spleen
    Sample buffer: 2% FCS, 0.5 mM EDTA in PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    lymphocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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