PE Anti-Ly76 antibody [TER-119] (ab93586)

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Flow Cytometry - PE Anti-Ly76 antibody [TER-119] (ab93586)

    Key features and details

    • PE Rat monoclonal [TER-119] to Ly76
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2b

    Overview

    • Product name

      PE Anti-Ly76 antibody [TER-119]
      See all Ly76 primary antibodies

    • Description

      PE Rat monoclonal [TER-119] to Ly76

    • Host species

      Rat

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue/ cell preparation - Mouse fetal liver cells

    • Positive control

      • BALB/c bone marrow cells

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab93586 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Target

    • Relevance

      Ly76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.

    • Cellular localization

      Plasma membrane

    • Database links

      • Alternative names

        • Ly 76 antibody
        • Lymphocyte antigen 76 antibody
        • TER 119 antibody
        • TER119 antibody

      Images

      • Flow Cytometry - PE Anti-Ly76 antibody [TER-119] (ab93586)
        Flow Cytometry - PE Anti-Ly76 antibody [TER-119] (ab93586)

        Flow cytometry of C57Bl/6 bone marrow cells labeling Ly76 with ab93586 at 0.5µg (solid line) or Rat IgG2b isotype control at 0.5µg (dashed line).

      References (2)

      Publishing research using ab93586? Please let us know so that we can cite the reference in this datasheet.

      ab93586 has been referenced in 2 publications.

      • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). PubMed: 10848813
      • Vannucchi AM  et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95:2559-68 (2000). PubMed: 10753835

      Customer reviews and Q&As

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      Flow Cytometry abreview for Anti-Ly76 antibody [TER-119] (Phycoerythrin)

       Excellent
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (Erythrocytes)
      Specification
      Erythrocytes
      Preparation
      Cell harvesting/tissue preparation method: Isolation of purified erythrocytes from whole blood using percoll gradient
      Sample buffer: PBS with 5mM EDTA
      Fixation
      Unfixed
      Permeabilization
      No
      Gating Strategy
      live cell population of purified erythrocytes gated on the basis of FSC/SSC. P2 gating for positive cell population was set so that <1% of the negative cell population was PE positive.
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 25 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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