For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    pe-m6pr-cation-independent-antibody-mem-238-ab77135.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • PE Mouse monoclonal [MEM-238] to M6PR (cation independent)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-M6PR (cation independent) antibody [MEM-238]
    See all M6PR (cation independent) primary antibodies
  • Description

    PE Mouse monoclonal [MEM-238] to M6PR (cation independent)
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant Vaccinia virus encoding CD222.

  • Epitope

    Ab77135 recognizes an epitope between domains 2 and 5 of CD222
  • Positive control

    • Human blood cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: 0.2% BSA, 0.2% PBS

    0.2% BSA (high-grade protease free)
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-238
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [MEM-238] (ab187589)
    • Biotin Anti-M6PR (cation independent) antibody [MEM-238] (ab239242)
    • FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
    • Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)
  • Isotype control

    • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

Target

  • Function

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • Sequence similarities

    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • Domain

    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • Cellular localization

    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Target information above from: UniProt accession P11717 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3482 Human
    • Omim: 147280 Human
    • SwissProt: P11717 Human
    • Unigene: 487062 Human
    • Unigene: 673278 Human
    • Alternative names

      • 300 kDa mannose 6 phosphate receptor antibody
      • 300 kDa mannose 6-phosphate receptor antibody
      • Cation independent mannose 6 phosphate receptor antibody
      • Cation-independent mannose-6-phosphate receptor antibody
      • CD222 antibody
      • CD222 antigen antibody
      • CI Man 6 P receptor antibody
      • CI Man-6-P receptor antibody
      • CI MPR antibody
      • CI-M6PR antibody
      • CI-MPR antibody
      • CIMPR antibody
      • IGF 2 receptor antibody
      • IGF 2R antibody
      • IGF II receptor antibody
      • IGF-II receptor antibody
      • IGF2 receptor antibody
      • Igf2r antibody
      • Insulin like growth factor 2 receptor antibody
      • Insulin like growth factor II receptor antibody
      • Insulin-like growth factor 2 receptor antibody
      • Insulin-like growth factor II receptor antibody
      • M6P R antibody
      • M6P/IGF2 receptor antibody
      • M6P/IGF2R antibody
      • M6PR antibody
      • mannose 6 phosphate receptor antibody
      • mannose 6 phosphate receptor, cation independent antibody
      • MPR 300 antibody
      • MPR300 antibody
      • MPRI antibody
      • MPRI_HUMAN antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab77135? Please let us know so that we can cite the reference in this datasheet.

    ab77135 has been referenced in 1 publication.

    • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). PubMed: 12189157

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I am inquiring about the anti-Mannose 6-Phosphate receptor antibody concentration (100 tests tube, xx mg/mL(?)) and what is the recommended expiry for this antibody when stored @ 4C?

    Thank you!

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2012

    Answer

    Thank you for contacting Abcam regarding ab77135.

    Regarding the expiration date for this antibody, when stored at 4oC, it has an expected shelf life of up to 1 year.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.