PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)

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Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)

    Key features and details

    • PE Mouse monoclonal [MEM-238] to M6PR (cation independent)
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 Biotin FITC Unconjugated

    Overview

    • Product name

      PE Anti-M6PR (cation independent) antibody [MEM-238]
      See all M6PR (cation independent) primary antibodies

    • Description

      PE Mouse monoclonal [MEM-238] to M6PR (cation independent)

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Non human primates

    • Immunogen

      Recombinant Vaccinia virus encoding CD222.

    • Epitope

      Ab77135 recognizes an epitope between domains 2 and 5 of CD222

    • Positive control

      • Flow Cyt: Human blood cells

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab77135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Target

    • Function

      Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.

    • Sequence similarities

      Belongs to the MRL1/IGF2R family.
      Contains 1 fibronectin type-II domain.

    • Domain

      Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.

    • Cellular localization

      Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
    • Information by UniProt

    • Database links

      • Alternative names

        • 300 kDa mannose 6 phosphate receptor antibody
        • 300 kDa mannose 6-phosphate receptor antibody
        • Cation independent mannose 6 phosphate receptor antibody
        • Cation-independent mannose-6-phosphate receptor antibody
        • CD222 antibody
        • CD222 antigen antibody
        • CI Man 6 P receptor antibody
        • CI Man-6-P receptor antibody
        • CI MPR antibody
        • CI-M6PR antibody
        • CI-MPR antibody
        • CIMPR antibody
        • IGF 2 receptor antibody
        • IGF 2R antibody
        • IGF II receptor antibody
        • IGF-II receptor antibody
        • IGF2 receptor antibody
        • Igf2r antibody
        • Insulin like growth factor 2 receptor antibody
        • Insulin like growth factor II receptor antibody
        • Insulin-like growth factor 2 receptor antibody
        • Insulin-like growth factor II receptor antibody
        • M6P R antibody
        • M6P/IGF2 receptor antibody
        • M6P/IGF2R antibody
        • M6PR antibody
        • mannose 6 phosphate receptor antibody
        • mannose 6 phosphate receptor, cation independent antibody
        • MPR 300 antibody
        • MPR300 antibody
        • MPRI antibody
        • MPRI_HUMAN antibody
        see all

      Images

      • Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)
        Flow Cytometry - PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)

        Flow cytometry analysis (intracellular staining) of human peripheral blood with anti-CD222 (MEM-238) PE.

      References (1)

      Publishing research using ab77135? Please let us know so that we can cite the reference in this datasheet.

      ab77135 has been referenced in 1 publication.

      • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). PubMed: 12189157

      Customer reviews and Q&As

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      Question

      I am inquiring about the anti-Mannose 6-Phosphate receptor antibody concentration (100 tests tube, xx mg/mL(?)) and what is the recommended expiry for this antibody when stored @ 4C?

      Thank you!

      Read More
      Answer

      Thank you for contacting Abcam regarding ab77135.

      Regarding the expiration date for this antibody, when stored at 4oC, it has an expected shelf life of up to 1 year.

      I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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