PE Anti-M6PR (cation independent) antibody [MEM-238] (ab77135)
Key features and details
- PE Mouse monoclonal [MEM-238] to M6PR (cation independent)
- Suitable for: Flow Cyt (Intra), Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-M6PR (cation independent) antibody [MEM-238]
See all M6PR (cation independent) primary antibodies -
Description
PE Mouse monoclonal [MEM-238] to M6PR (cation independent) -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra), Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human M6PR (cation independent).
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Epitope
Ab77135 recognizes an epitope between domains 2 and 5 of CD222 -
Positive control
- Flow Cyt: Human blood cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 0.2% PBS
0.2% BSA (high-grade protease free) -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-238 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
Use 20 ul for 10^6 cells in a suspension or 100 ul of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. Use 20 ul for 10^6 cells in a suspension or 100 ul of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3482 Human
- Omim: 147280 Human
- SwissProt: P11717 Human
- Unigene: 487062 Human
- Unigene: 673278 Human
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Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab77135 has been referenced in 1 publication.
- Leksa V et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). PubMed: 12189157