PE Anti-Macrophage antibody [BM8] (ab125148)

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Flow Cytometry - PE Anti-Macrophage antibody [BM8] (ab125148)

    Key features and details

    • PE Rat monoclonal [BM8] to Macrophage
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Overview

    • Product name

      PE Anti-Macrophage antibody [BM8]
      See all Macrophage primary antibodies

    • Description

      PE Rat monoclonal [BM8] to Macrophage

    • Host species

      Rat

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

    • Positive control

      • Flow Cyt: C57Bl/6 bone marrow cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab125148 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt Use at an assay dependent concentration.

      ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

      Target

      • Relevance

        Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

      • Alternative names

        • macrophages antibody

      Images

      • Flow Cytometry - PE Anti-Macrophage antibody [BM8] (ab125148)
        Flow Cytometry - PE Anti-Macrophage antibody [BM8] (ab125148)

        C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (ab25482) and 0.25µg PE Anti-Mouse F4/80 Antigen (ab125148) (right panel) or 0.25µg PE Rat IgG2b isotype control (left panel).

      References (1)

      Publishing research using ab125148? Please let us know so that we can cite the reference in this datasheet.

      ab125148 has been referenced in 1 publication.

      • Leenen PJ  et al. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 174:5-19 (1994). PubMed: 8083537

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