RecombinantRabMAb

Recombinant PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)

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Flow Cytometry - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)
  • PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP603Y] to MALT1/MLT
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-MALT1/MLT antibody [EP603Y]
    See all MALT1/MLT primary antibodies

  • Description

    PE Rabbit monoclonal [EP603Y] to MALT1/MLT

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MALT1/MLT aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UDY8

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells

  • General notes

     This product was previously labelled as MALT1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab210982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50. This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.

  • Tissue specificity

    Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.

  • Involvement in disease

    Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.

  • Sequence similarities

    Belongs to the peptidase C14B family.
    Contains 1 death domain.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • Cellular localization

    Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.
  • Information by UniProt

  • Database links

    • Alternative names

      • Caspase like protein antibody
      • DKFZp434L132 antibody
      • IMD12 antibody
      • MALT 1 antibody
      • MALT associated translocation antibody
      • MALT lymphoma associated translocation antibody
      • MALT lymphoma-associated translocation antibody
      • Malt1 antibody
      • MALT1 paracaspase antibody
      • MALT1_HUMAN antibody
      • MLT 1 antibody
      • MLT antibody
      • MLT1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation gene 1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Mucosa-associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Paracaspase antibody
      • Paracaspase-1 antibody
      • PCASP1 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)
      Flow Cytometry - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)

      Overlay histogram showing HeLa cells stained with ab210982 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210982, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)
      Immunocytochemistry/ Immunofluorescence - PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)

      ab210982 staining MALT1/MLT in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210982 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)
      PE Anti-MALT1/MLT antibody [EP603Y] (ab210982)

    References (1)

    Publishing research using ab210982? Please let us know so that we can cite the reference in this datasheet.

    ab210982 has been referenced in 1 publication.

    • Kan B  et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). PubMed: 30446641

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