Recombinant PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)
![Flow Cytometry - PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)](https://www.abcam.com/ps/products/211/ab211691/Images/ab211691-264912-anti-map1lc3a-antibody-ep1983y-autophagosome-marker-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1983Y] to MAP1LC3A - Autophagosome Marker
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker
See all MAP1LC3A primary antibodies -
Description
PE Rabbit monoclonal [EP1983Y] to MAP1LC3A - Autophagosome Marker -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human MAP1LC3A aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9H492 -
Positive control
- Flow Cyt: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1983Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab211691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy related protein LC3 A antibody
- Autophagy related ubiquitin like modifier LC3 A antibody
see all
Images
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Flow Cytometry - PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)Overlay histogram showing SH-SY5Y cells stained with ab211691 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211691, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. -
![PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)](https://www.abcam.com/ps/products/211/ab211691/Images/ab211691-3-benefits-of-recombinant-antibodies.png)
PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)
References (0)
ab211691 has not yet been referenced specifically in any publications.
