Recombinant PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker (ab211691)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1983Y] to MAP1LC3A - Autophagosome Marker
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-MAP1LC3A antibody [EP1983Y] - Autophagosome Marker
See all MAP1LC3A primary antibodies -
Description
PE Rabbit monoclonal [EP1983Y] to MAP1LC3A - Autophagosome Marker -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1983Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy related protein LC3 A antibody
- Autophagy related ubiquitin like modifier LC3 A antibody
see all
Images
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Overlay histogram showing SH-SY5Y cells stained with ab211691 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211691, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab211691 has not yet been referenced specifically in any publications.