PE Anti-MCK10/NEP antibody [51D6] (ab253251)
![Flow Cytometry - PE Anti-MCK10/NEP antibody [51D6] (ab253251)](https://www.abcam.com/ps/products/253/ab253251/Images/ab253251-343884-antmck10nepantibody51d6-flow-cytometry-mcf7-human.jpg "Flow Cytometry - PE Anti-MCK10/NEP antibody [51D6] (ab253251)")
Key features and details
- PE Mouse monoclonal [51D6] to MCK10/NEP
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG3
Overview
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Product name
PE Anti-MCK10/NEP antibody [51D6]
See all MCK10/NEP primary antibodies -
Description
PE Mouse monoclonal [51D6] to MCK10/NEP -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to MCK10/NEP. MCK10/NEP-transfected NIH/3T3 cells.
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Positive control
- Flow Cyt: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 8.00
Preservative: 0.0975% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
51D6 -
Isotype
IgG3 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Or 10 μL per 100 μL whole blood. |
Target
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Function
May be involved in cell-cell interactions and recognition. -
Tissue specificity
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain. -
Domain
The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates. -
Cellular localization
Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 780 Human
- Omim: 600408 Human
- SwissProt: Q08345 Human
- Unigene: 631988 Human
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Alternative names
- CAK antibody
- CD 167 antibody
- CD167 antibody
see all
Images
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Flow Cytometry - PE Anti-MCK10/NEP antibody [51D6] (ab253251)
Flow cytometric anlysis of MCF7 (human breast adenocarcinoma cell line) cells using ab253251. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253251 has not yet been referenced specifically in any publications.
