Key features and details
- PE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Product namePE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
See all MHC Class 1 H2 Db/H2-D1 primary antibodies
DescriptionPE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1.
EpitopeThis antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.
- Flow cyt: Mouse BALB/c splenocytes.
This product was previously labelled as MHC Class 1 H2 Db
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent.
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab25547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
Cellular localizationCell Membrane; single pass type I membrane protein
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
ab25547 has been referenced in 1 publication.
- Hoerter JA et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). PubMed: 23940257