RecombinantRabMAb

Recombinant PE Anti-MMP1 antibody [EP1247Y] (ab209574)

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Flow Cytometry - PE Anti-MMP1 antibody [EP1247Y] (ab209574)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-MMP1 antibody [EP1247Y] (ab209574)
  • PE Anti-MMP1 antibody [EP1247Y] (ab209574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP1247Y] to MMP1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-MMP1 antibody [EP1247Y]
    See all MMP1 primary antibodies

  • Description

    PE Rabbit monoclonal [EP1247Y] to MMP1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MMP1 aa 100-200. The exact sequence is proprietary.
    Database link: P03956

  • Positive control

    • Flow Cyt: SH-SY5Y cells ICC/IF: SKNSH cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.
ICC/IF 1/100.

This product gave a positive signal in SKNSH cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • Domain

    There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Post-translational
    modifications

    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • 27 kDa interstitial collagenase antibody
      • CLG antibody
      • CLGN antibody
      • collagenase, fibroblast antibody
      • collagenase, interstitial antibody
      • Fibroblast collagenase antibody
      • Interstitial collagenase antibody
      • Matrix metallopeptidase 1 (interstitial collagenase) antibody
      • Matrix metalloprotease 1 antibody
      • Matrix Metalloproteinase 1 antibody
      • Matrix metalloproteinase-1 antibody
      • MMP 1 antibody
      • MMP-1 antibody
      • MMP1 antibody
      • MMP1_HUMAN antibody
      • OTTHUMP00000045866 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-MMP1 antibody [EP1247Y] (ab209574)
      Flow Cytometry - PE Anti-MMP1 antibody [EP1247Y] (ab209574)

      Overlay histogram showing SH-SY5Y cells stained with ab209574 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209574, 1/5000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - PE Anti-MMP1 antibody [EP1247Y] (ab209574)
      Immunocytochemistry/ Immunofluorescence - PE Anti-MMP1 antibody [EP1247Y] (ab209574)

      ab209574 staining MMP1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209574 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

    • PE Anti-MMP1 antibody [EP1247Y] (ab209574)
      PE Anti-MMP1 antibody [EP1247Y] (ab209574)

    References (0)

    Publishing research using ab209574? Please let us know so that we can cite the reference in this datasheet.

    ab209574 has not yet been referenced specifically in any publications.

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