For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pe-mouse-igg1-b116-isotype-control-ab91357.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Isotype/Loading Controls Isotype Controls Mouse
Share by email

PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

  • Datasheet
Q&A (3)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Primary
Product image
Alexa Fluor® 647 Anti-HMGB1 antibody [EPR3507] (ab195011)
Primary
Product image
PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)

View more associated products

Overview

  • Product name

    PE Mouse IgG1 [B11/6] - Isotype Control
    See all Mouse isotype controls
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Immunogen

    A synthetic hapten

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    B11/6
  • Isotype

    IgG1
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Alternative Versions

    • PerCP Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab118658)
    • CF405M Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab126026)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Our Abpromise guarantee covers the use of ab91357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

ab91357 is used as a isotype negative control.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab91357? Please let us know so that we can cite the reference in this datasheet.

    ab91357 has been referenced in 3 publications.

    • Quayle LA  et al. Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention. Clin Exp Metastasis 35:831-846 (2018). PubMed: 30377878
    • Sun W  et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). PubMed: 29201913
    • Sondag GR  et al. Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling. Exp Mol Med 48:e257 (2016). IP . PubMed: 27585719

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Inquiry: Dear Sir/Madam I have a question about the antibodies that I am going to purchase. I am going to purchase a Anti-6X His tag (PE)antibody (ab72467) for my Flow cytometry analysis on bacterial cells (Salmonella Typhimurium). I just want to find the 1. Ab concentration of the product, 2. the F:P ratio, and 3. a suitible isotype control. I've narrow down the isotype control to two possible mAb (PE), which are (ab1280) and (ab91357). Is it possible for these isotype controls to react with Salmonella typhimurium proteins? are they raised to against any bacterial proteins? I's also like to know their Ab concentration and F:P ratio too. Thanks very much

    Read More

    Abcam community

    Verified customer

    Asked on Jun 29 2012

    Answer

    Thank you for contacting us and your interest in our products.
    In answer to your questions:
    1. Ab concentration of anti-6X His tag (PE), ab72467
    The concentration of this antibody is 69 ug/mL.
    2. The F:P ratio of the antibody
    The F:P ratio of ab72467 is 1:1
    3. A suitable isotype control
    I think either of the antibodies you have suggested would be suitable as an isotype control. The first, ab1280, is supplied at a concentration of 23 ug/mL and the F:P ratio is lot dependent. The current lot is a ratio of 0.9. The second antibody, ab91357, is supplied at a concentration of 7.5 ug/mL and the F:P ratio is between 1-2. It is very unlikely that either of these isotype controls will cross react with your Salmonella typhimurium however as we have not tested this ourselves we would be able to guarantee it.
    I hope this information has been of help, if you have any further questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Jun 29 2012

    Question

    Hi Technical support!



    Please refer to an email below from one of our customers and I hope you may be able to help out:



    I am going to purchase a Anti-6X His tag antibody (ab72467) for my Flow cytometry analysis. I'm just wondering if Abcam also supply other antibodies which can be used for my analysis as an Isotype control (Negative control). Should be something that doesn't react with my Histidine tag, but also has the Phycoerythrin conjugate.



    Thank you heaps heaps J



    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2012

    Answer

    Thank you for your inquriy.

    I am happy to confirm that we offer a isotype control for ab72467 that is also PE conjugated:

    ab91357

    https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html (or use the following: https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html).

    ab91357 was not specifically tested to not bind to HIS tags. But we believe it is very unlikely since the immunogen used for this antibody was a vegetal immunogen.

    I hope this information is helpful, and wish your customer good luck with their experiments.

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2012

    Question

    P {margin-top:0;margin-bottom:0;} Hi, I would need a isotype control for Anti-CD11b (ED8) PE Cat # ab33816-50 for flowcytometry. Can you recommend me a suitable one?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2011

    Answer

    Thank you for your enquiry. An example of an isotype control you can use for flow with ab33816 is ab91357. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.