PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

Q&A (3)References (5)

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Overview

  • Product name

    PE Mouse IgG1 [B11/6] - Isotype Control
    See all Mouse isotype controls

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    A synthetic hapten

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    B11/6

  • Isotype

    IgG1

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 20µl for 106 cells.

ab91357 is used as a isotype negative control.
Notes
Flow Cyt
Use 20µl for 106 cells.

ab91357 is used as a isotype negative control.

References (5)

Publishing research using ab91357? Please let us know so that we can cite the reference in this datasheet.

ab91357 has been referenced in 5 publications.

  • Lotsberg ML  et al. AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells. J Thorac Oncol 15:973-999 (2020). PubMed: 32018052
  • Avril M  et al. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption. Sci Rep 9:13142 (2019). PubMed: 31511575
  • Quayle LA  et al. Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention. Clin Exp Metastasis 35:831-846 (2018). PubMed: 30377878
  • Sun W  et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). PubMed: 29201913
  • Sondag GR  et al. Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling. Exp Mol Med 48:e257 (2016). IP . PubMed: 27585719

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

Inquiry: Dear Sir/Madam I have a question about the antibodies that I am going to purchase. I am going to purchase a Anti-6X His tag (PE)antibody (ab72467) for my Flow cytometry analysis on bacterial cells (Salmonella Typhimurium). I just want to find the 1. Ab concentration of the product, 2. the F:P ratio, and 3. a suitible isotype control. I've narrow down the isotype control to two possible mAb (PE), which are (ab1280) and (ab91357). Is it possible for these isotype controls to react with Salmonella typhimurium proteins? are they raised to against any bacterial proteins? I's also like to know their Ab concentration and F:P ratio too. Thanks very much

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Answer

Thank you for contacting us and your interest in our products.
In answer to your questions:
1. Ab concentration of anti-6X His tag (PE), ab72467
The concentration of this antibody is 69 ug/mL.
2. The F:P ratio of the antibody
The F:P ratio of ab72467 is 1:1
3. A suitable isotype control
I think either of the antibodies you have suggested would be suitable as an isotype control. The first, ab1280, is supplied at a concentration of 23 ug/mL and the F:P ratio is lot dependent. The current lot is a ratio of 0.9. The second antibody, ab91357, is supplied at a concentration of 7.5 ug/mL and the F:P ratio is between 1-2. It is very unlikely that either of these isotype controls will cross react with your Salmonella typhimurium however as we have not tested this ourselves we would be able to guarantee it.
I hope this information has been of help, if you have any further questions, please do not hesitate to ask.

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Question

Hi Technical support!



Please refer to an email below from one of our customers and I hope you may be able to help out:



I am going to purchase a Anti-6X His tag antibody (ab72467) for my Flow cytometry analysis. I'm just wondering if Abcam also supply other antibodies which can be used for my analysis as an Isotype control (Negative control). Should be something that doesn't react with my Histidine tag, but also has the Phycoerythrin conjugate.



Thank you heaps heaps J



Kind regards,

Read More
Answer

Thank you for your inquriy.

I am happy to confirm that we offer a isotype control for ab72467 that is also PE conjugated:

ab91357

https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html (or use the following: https://www.abcam.com/PE-Mouse-IgG1-B116-Isotype-Control-ab91357.html).

ab91357 was not specifically tested to not bind to HIS tags. But we believe it is very unlikely since the immunogen used for this antibody was a vegetal immunogen.

I hope this information is helpful, and wish your customer good luck with their experiments.

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Question

P {margin-top:0;margin-bottom:0;} Hi, I would need a isotype control for Anti-CD11b (ED8) PE Cat # ab33816-50 for flowcytometry. Can you recommend me a suitable one?

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Answer

Thank you for your enquiry. An example of an isotype control you can use for flow with ab33816 is ab91357. I hope this is helpful. Please contact me again if you have any further questions.

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