Recombinant PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)
![Flow Cytometry - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)](https://www.abcam.com/ps/products/210/ab210418/Images/ab210418-261616-ab210418FLOW1.jpg "Flow Cytometry - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Musashi 1 / Msi1 antibody [EP1302]
See all Musashi 1 / Msi1 primary antibodies -
Description
PE Rabbit monoclonal [EP1302] to Musashi 1 / Msi1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Chicken, Human, Quail
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Immunogen
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Positive control
- Flow Cyt: Neuro 2A (differentiated).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1302 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab210418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/2500. |
Target
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Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
Tissue specificity
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. -
Sequence similarities
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The first RNA recognition motif binds more strongly to RNA compared to the second one. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4440 Human
- Entrez Gene: 17690 Mouse
- Omim: 603328 Human
- SwissProt: O43347 Human
- SwissProt: Q61474 Mouse
- Unigene: 158311 Human
- Unigene: 5077 Mouse
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Alternative names
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
see all
Images
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Flow Cytometry - PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)
Overlay histogram showing Neuro 2A (differentiated) cells stained with ab210418 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210418, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)](https://www.abcam.com/ps/products/210/ab210418/Images/ab210418-2-benefits-of-recombinant-antibodies.png)
PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)
Datasheets and documents
Certificate of Compliance
References (0)
ab210418 has not yet been referenced specifically in any publications.
