PE Anti-N Cadherin antibody [8C11] (ab93525)
![Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (ab93525)](https://www.abcam.com/ps/products/93/ab93525/Images/ab93525-108563-anti-n-cadherin-antibody-8c11-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (ab93525)")
Key features and details
- PE Mouse monoclonal [8C11] to N Cadherin
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-N Cadherin antibody [8C11]
See all N Cadherin primary antibodies -
Description
PE Mouse monoclonal [8C11] to N Cadherin -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Bacterial fusion protein corresponding to the extracellular domain of Human N Cadherin.
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Positive control
- HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8C11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab93525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1000 Human
- Omim: 114020 Human
- SwissProt: P19022 Human
- Unigene: 464829 Human
- Unigene: 606106 Human
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Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Images
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Flow Cytometry - PE Anti-N Cadherin antibody [8C11] (ab93525)Staining of HeLa cells with PE Mouse IgG1 kappa Isotype Control (blue histogram), or ab93525. Total viable cells were used for analysis.
References (2)
ab93525 has been referenced in 2 publications.
- Wahl JK et al. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem 278:17269-76 (2003). WB . PubMed: 12604612
- Puch S et al. N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. J Cell Sci 114:1567-77 (2001). Flow Cyt . PubMed: 11282032
