Recombinant PE Anti-Nectin 2 antibody [EPR6717] (ab237121)
![Flow Cytometry - PE Anti-Nectin 2 antibody [EPR6717] (ab237121)](https://www.abcam.com/ps/products/237/ab237121/Images/ab237121-2-RabbitmonoclonalEPR6717toNectin2RPEHepG2cellsflowcytometry333.jpg "Flow Cytometry - PE Anti-Nectin 2 antibody [EPR6717] (ab237121)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR6717] to Nectin 2
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Nectin 2 antibody [EPR6717]
See all Nectin 2 primary antibodies -
Description
PE Rabbit monoclonal [EPR6717] to Nectin 2 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody does not recognize Isoform Alpha of human Nectin 2.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.
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Positive control
- Flow Cyt: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab237121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Probable cell adhesion protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5819 Human
- Omim: 600798 Human
- SwissProt: Q92692 Human
- Unigene: 655455 Human
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Alternative names
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
Images
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Flow Cytometry - PE Anti-Nectin 2 antibody [EPR6717] (ab237121)
Overlay histogram showing HepG2 cells stained with ab237121 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab237121) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HepG2 cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![PE Anti-Nectin 2 antibody [EPR6717] (ab237121)](https://www.abcam.com/ps/products/237/ab237121/Images/ab237121-3-benefits-of-recombinant-antibodies.png)
PE Anti-Nectin 2 antibody [EPR6717] (ab237121)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237121 has not yet been referenced specifically in any publications.
