PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)
![Flow Cytometry - PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)](https://www.abcam.com/ps/products/125/ab125159/Images/ab125159-282334-anti-neutrophil-antibody-nimp-r14-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)")
Key features and details
- PE Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
-
Product name
PE Anti-Neutrophil antibody [NIMP-R14]
See all Neutrophil primary antibodies -
Description
PE Rat monoclonal [NIMP-R14] to Neutrophil -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Purified BALB/c Mouse neutrophils.
-
Epitope
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
Positive control
- Mouse spleen tissue; Mouse neutrophils.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NIMP-R14 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab125159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 105 cells. ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
-
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 110453 Mouse
-
Alternative names
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
Images
-
Flow Cytometry - PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)
Detection of Ly-6G/-6C in RAW cells. Red, black and blue line represent the isotype control, cells only and ab125159 at 10 μg/ml, respectively.
Protocols
Datasheets and documents
References (4)
ab125159 has been referenced in 4 publications.
- Menten-Dedoyart C et al. Neutrophil extracellular traps entrap and kill Borrelia burgdorferi sensu stricto spirochetes and are not affected by Ixodes ricinus tick saliva. J Immunol 189:5393-401 (2012). ICC/IF ; Mouse . PubMed: 23109724
- de Vries B et al. Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils. J Immunol 170:3883-9 (2003). PubMed: 12646657
- Van Lent PL et al. Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritis in mice. J Leukoc Biol 61:267-78 (1997). PubMed: 9060449
- Salling AL [Organization "Moder Jord" goes in for home delivery]. Sygeplejersken 76:11 (1976). PubMed: 1051039
