PE Anti-NUR77 antibody [12.14] (ab95659)
![Flow Cytometry - PE Anti-NUR77 antibody [12.14] (ab95659)](https://www.abcam.com/ps/products/95/ab95659/Images/NUR77-Primary-antibodies-ab95659-1.jpg "Flow Cytometry - PE Anti-NUR77 antibody [12.14] (ab95659)")
Key features and details
- PE Mouse monoclonal [12.14] to NUR77
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-NUR77 antibody [12.14]
See all NUR77 primary antibodies -
Description
PE Mouse monoclonal [12.14] to NUR77 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length mouse Nur77 fusion protein
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Positive control
- Mouse thymocytes stimulated with PMA and ionomycin for two hours.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
12.14 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 105-8 cells. in a final volume of 100 µL |
Target
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Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. -
Tissue specificity
Fetal muscle and adult liver, brain and thyroid. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 15370 Mouse
- SwissProt: P12813 Mouse
- Unigene: 119 Mouse
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Alternative names
- Early response protein NAK1 antibody
- GFRP 1 antibody
- GFRP antibody
see all
Images
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Flow Cytometry - PE Anti-NUR77 antibody [12.14] (ab95659)Flow cytometry images showing staining of unstimulated (left) and 2-hr PMA and Ionomycin stimulated (right) C57BL/6 thymocytes with an Anti-Mouse CD4 APC antibody, followed by intracellular staining with 0.5 µg of ab95659 using a Foxp3 Staining Buffer Set. Total viable cells were used for analysis.
Datasheets and documents
References (0)
ab95659 has not yet been referenced specifically in any publications.
