PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656)
![Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656)](https://www.abcam.com/ps/products/95/ab95656/Images/ab95656-109919-anti-cd252-antibody-rm134l-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656)")
Key features and details
- PE Rat monoclonal [RM134L] to OX40L/TNFSF4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-OX40L/TNFSF4 antibody [RM134L]
See all OX40L/TNFSF4 primary antibodies -
Description
PE Rat monoclonal [RM134L] to OX40L/TNFSF4 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse CD252 protein
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Positive control
- Anti-Mouse IgM and Anti-Mouse CD40 stimulated Mouse BALB/c splenocytes
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General notes
The RM134L monoclonal antibody inhibits the costimulatory activity of Mouse CD252 transfected cells for anti-CD3-stimulated T cell proliferation.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The RM134L monoclonal antibody inhibits the costimulatory activity of Mouse CD252 transfected cells for anti-CD3-stimulated T cell proliferation. -
Clonality
Monoclonal -
Clone number
RM134L -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25-0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended. |
Target
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Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. -
Involvement in disease
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22164 Mouse
- SwissProt: P43488 Mouse
- Unigene: 4994 Mouse
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Alternative names
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
see all
Images
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Flow Cytometry - PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656)
Flow cytometry staining of 3-day unstimulated (left) and 3-day Anti-Mouse IgM and Anti-Mouse CD40 stimulated (right) BALB/c splenocytes with 0.25 µg of Rat IgG2b Isotype Control PE (open histogram) or 0.25 µg of ab95656 (filled histogram). Total viable cells were used for analysis.
References (3)
ab95656 has been referenced in 3 publications.
- Tsukada N et al. Blockade of CD134 (OX40)-CD134L interaction ameliorates lethal acute graft-versus-host disease in a murine model of allogeneic bone marrow transplantation. Blood 95:2434-9 (2000). PubMed: 10733518
- Akiba H et al. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med 191:375-80 (2000). PubMed: 10637281
- Akiba H et al. CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. J Immunol 162:7058-66 (1999). PubMed: 10358148
