PE Anti-OX40L/TNFSF4 antibody [RM134L] (ab95656)
Key features and details
- PE Rat monoclonal [RM134L] to OX40L/TNFSF4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-OX40L/TNFSF4 antibody [RM134L]
See all OX40L/TNFSF4 primary antibodies -
Description
PE Rat monoclonal [RM134L] to OX40L/TNFSF4 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse OX40L/TNFSF4. Native protein
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Positive control
- Anti-Mouse IgM and Anti-Mouse CD40 stimulated Mouse BALB/c splenocytes
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General notes
The RM134L monoclonal antibody inhibits the costimulatory activity of Mouse CD252 transfected cells for anti-CD3-stimulated T cell proliferation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The RM134L monoclonal antibody inhibits the costimulatory activity of Mouse CD252 transfected cells for anti-CD3-stimulated T cell proliferation. -
Clonality
Monoclonal -
Clone number
RM134L -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.25-0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µl is recommended. |
Notes |
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Flow Cyt
Use 0.25-0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended. |
Target
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Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. -
Involvement in disease
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22164 Mouse
- SwissProt: P43488 Mouse
- Unigene: 4994 Mouse
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Alternative names
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab95656 has been referenced in 3 publications.
- Tsukada N et al. Blockade of CD134 (OX40)-CD134L interaction ameliorates lethal acute graft-versus-host disease in a murine model of allogeneic bone marrow transplantation. Blood 95:2434-9 (2000). PubMed: 10733518
- Akiba H et al. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med 191:375-80 (2000). PubMed: 10637281
- Akiba H et al. CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. J Immunol 162:7058-66 (1999). PubMed: 10358148