PE Anti-P Glycoprotein antibody [UIC2] (ab269309)
![Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab269309)](https://www.abcam.com/ps/products/269/ab269309/Images/ab269309-1-AntiCD243PEantibodyHumanflow-cytometry.jpg "Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab269309)")
Key features and details
- PE Mouse monoclonal [UIC2] to P Glycoprotein
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-P Glycoprotein antibody [UIC2]
See all P Glycoprotein primary antibodies -
Description
PE Mouse monoclonal [UIC2] to P Glycoprotein -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab269309 recognizes an extracellular epitope on P-Glycoprotein.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus. NIH 3T3 cells transfected with human P Glycoprotein cDNA.
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Positive control
- Flow cyt: HepG2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
UIC2 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab269309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Or 100 μL of whole blood. |
Target
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Function
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. -
Tissue specificity
Expressed in liver, kidney, small intestine and brain. -
Involvement in disease
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5243 Human
- Omim: 171050 Human
- SwissProt: P08183 Human
- Unigene: 489033 Human
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Alternative names
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
see all
Images
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Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab269309)Flow cytometry analysis (surface staining) of HepG2 cells labeling CD243 with ab269309.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab269309 has not yet been referenced specifically in any publications.
