PE Anti-P Glycoprotein antibody [UIC2] (ab93590)

Submit a review Q&A (4)References (7)
Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab93590)

    Key features and details

    • PE Mouse monoclonal [UIC2] to P Glycoprotein
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Overview

    • Product name

      PE Anti-P Glycoprotein antibody [UIC2]
      See all P Glycoprotein primary antibodies

    • Description

      PE Mouse monoclonal [UIC2] to P Glycoprotein

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Mouse Balb/c 3T3 fibroblasts transfected with Human P Glycoprotein cDNA

    • Positive control

      • 8226 DOX40 and 8226/S cell lines

    • General notes


      ab93590 has been reported to inhibit MDR1-mediated efflux.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab93590 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 5µl for 106 cells.

    Use 5 µl/test for 105-8 cells in 100µl.

     

     

     

     

    ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

    • Tissue specificity

      Expressed in liver, kidney, small intestine and brain.

    • Involvement in disease

      Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ABC20 antibody
        • ABCB1 antibody
        • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
        • ATP-binding cassette sub-family B member 1 antibody
        • CD243 antibody
        • CLCS antibody
        • Colchicin sensitivity antibody
        • Doxorubicin resistance antibody
        • GP170 antibody
        • MDR1 antibody
        • MDR1_HUMAN antibody
        • Multidrug resistance 1 antibody
        • Multidrug resistance protein 1 antibody
        • P glycoprotein 1 antibody
        • P gp antibody
        • P-glycoprotein 1 antibody
        • PGY1 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab93590)
        Flow Cytometry - PE Anti-P Glycoprotein antibody [UIC2] (ab93590)
        Staining of the 8226/S (blue histogram) and 8226 DOX40 (purple histogram) cell lines with PE Mouse IgG2a, K Isotype Control (left) or ab93590 (right). Total viable cells were used for analysis.

      References (7)

      Publishing research using ab93590? Please let us know so that we can cite the reference in this datasheet.

      ab93590 has been referenced in 7 publications.

      • Guo HH  et al. Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases. Nat Commun 10:1981 (2019). PubMed: 31040273
      • Rafiee R  et al. ABCB1 SNP predicts outcome in patients with acute myeloid leukemia treated with Gemtuzumab ozogamicin: a report from Children's Oncology Group AAML0531 Trial. Blood Cancer J 9:51 (2019). PubMed: 31113932
      • Lopes A  et al. Chiral Thioxanthones as Modulators of P-glycoprotein: Synthesis and Enantioselectivity Studies. Molecules 23:N/A (2018). PubMed: 29534440
      • Wang CW  et al. 7-ketocholesterol and 27-hydroxycholesterol decreased doxorubicin sensitivity in breast cancer cells: estrogenic activity and mTOR pathway. Oncotarget 8:66033-66050 (2017). PubMed: 29029490
      • Huang XC  et al. Parguerenes: Marine red alga bromoditerpenes as inhibitors of P-glycoprotein (ABCB1) in multidrug resistant human cancer cells. Biochem Pharmacol 85:1257-68 (2013). PubMed: 23415901
      • Chaudhary PM  et al. Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood 80:2735-9 (1992). PubMed: 1360267
      • Mechetner EB & Roninson IB Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A 89:5824-8 (1992). PubMed: 1352877

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Question

      Inquiry: I am going to buy the following antibody from Abcam Anti-P Glycoprotein antibody [UIC2] (Phycoerythrin) (ab93590). It states on the data sheet that the isotype control used for this is a mouse IgG2a kappa but the only one i can find on your site is conjugated to PE/Cy7 and not just PE. Can you compare between these dyes on the flow cytometer or do you have a PE conjugated mouse IgG2a I can use?

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      Answer

      Thank you for contacting us.

      Please buy ab91363.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Hi Tech team, how are you today?



      We have a customer who wants to know whether ab93590 and ab1282 can be used to detect Pgp on cancer cells by flow cytometry.



      Looking forward to your response soon.



      Thanks, have a nice day!



      Kind regards,



      David Yi



      Customer Service and Technical Support - Davidy@sapphirebioscience.com

      ________________________

      Sapphire Bioscience Pty. Ltd.

      126 Cope Street │ Waterloo NSW 2017 │ Australia

      Ph: +61 2 9698 2022 │ Fax: +61 2 9698 1022 | http://www.sapphirebioscience.com/

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      Answer

      Thank you for your inquiry.

      I am happy to confirm that ab93590 (the isotype control for it (ab1282) will not bind anything specifically, but mark the background staining) is suitable for flow cytometry. It will recognise P-glycoprotein 1 (Pgp) if it is expressed by the cancer cell line your customer is using.

      I suggest to run cells from 8226 DOX40 and 8226/S cell lines as a positive control.

      I hope this information is helpful for your customer and wish them good luck for their research.

      Have a nice day too!

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      Question

      Dear Sir/Madam,

      I would like to buy an antibody against to P-glycoprotein to detect over-expressing of P-gp on cancer cells using flow cytometry. Could you please let me know these below two products from your company are the right choice for my experiment?
      ab93590 for detecting P-gp and ab1282 as an isotype control.

      If you have any good advice please tell me. I would like to purchase the products as soon as possible.

      Thank you for your time.

      Read More
      Answer

      Thank you for your enquiry and your interest.

      I can confirm that ab93590has been testedin Flow cytometry and ab1282 can be used as isotype control.

      We also have other primary antibodies against P Glycoprotein, here are some non-conjugated:

      - ab3366, ab80594, ab10333

      and some labelled to fluorochromes:

      - ab66250, ab93590

      I hope this helps and if I can assist further, please do not hesitate to contact me.

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      Question

      phone call: concentration and indiciations for dilution

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      Answer

      Merci pour votre enquête ainsi que pour votre patience pendant que je contactais le laboratoire. Le laboratoire m'a pu répondre que la concentration de cet anticorps est d'environ 0.5ug par 5ul. Donc ceci fait 0.1mg/ml. Le laboratoire à testé 5ul de cet anticorps sur 1 million de cellules dans 100ul. La gamme aussi large de la recommandation sur notre fiche technique est simplement du au fait de la gamme des cellules aussi large qui peuvent être marqué avec cet anticorps. J'espère que ceci répond à votre question. Veuillez ne pas hésiter à nous recontacter si vous avez des autres question. Je vous souhaite bon succès avec votre recherche.   

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