PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

Reviews (2)Q&A (2)References (16)
Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

    Key features and details

    • PE Mouse monoclonal [C-11] to pan Cytokeratin
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-pan Cytokeratin antibody [C-11]
      See all pan Cytokeratin primary antibodies

    • Description

      PE Mouse monoclonal [C-11] to pan Cytokeratin

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

    • Tested applications

      Suitable for: Flow Cyt (Intra)more details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human pan Cytokeratin. Keratin-enriched preparation from human epidermoid carcinoma cell line A431

    • Positive control

      • Flow Cyt (Intra): HT-29 cells

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.

    • Clonality

      Monoclonal

    • Clone number

      C-11

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    Use a concentration of 1 µg/ml.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    Notes
    Flow Cyt (Intra)
    Use a concentration of 1 µg/ml.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Relevance

      Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • pan ck antibody
        • pan-ck antibody
        • panck antibody

      Images

      • Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
        Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

        Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460. 

        Recommended conc. 0.5 µg/ml 

        Overlay with Isotype mouse IgG1 control. 

         

         

      References (16)

      Publishing research using ab52460? Please let us know so that we can cite the reference in this datasheet.

      ab52460 has been referenced in 16 publications.

      • Korangath P & Ivkov R Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles. Bio Protoc 10:e3822 (2020). PubMed: 33659474
      • Cheng KS  et al. ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells. Theranostics 10:11026-11048 (2020). PubMed: 33042268
      • Karin O  et al. Senescent cell turnover slows with age providing an explanation for the Gompertz law. Nat Commun 10:5495 (2019). PubMed: 31792199
      • Singh M  et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res 79:2962-2977 (2019). PubMed: 30952634
      • Sun Y  et al. CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients. EBioMedicine 46:133-149 (2019). PubMed: 31375425
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Flow Cytometry abreview for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)

       Excellent
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (kidney, epithelial)
      Permeabilization
      Yes - Biolegend Intracellular Staining Permeabilization Wash Buffer
      Gating Strategy
      all live cells
      Specification
      kidney, epithelial
      Preparation
      Cell harvesting/tissue preparation method: primary cells passaged and trypisinzed from flask at p2
      Sample buffer: Biolegend Cell Staining Buffer (paraformaldehyde based)
      Fixation
      Biolegend Flurofix
      Read More

      MISS. Kara Shumansky

      Verified customer

      Submitted Mar 04 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (kidney, proximal tubule)
      Permeabilization
      Yes - 1% triton
      Specification
      kidney, proximal tubule
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      MISS. Kara Shumansky

      Verified customer

      Submitted Feb 25 2019

      Question


      Request you to please let me know whether abcam
      can supply the following antibodies (reactivity:
      human)
      1. Fluorescein isothiocyanate (FITC)-conjugated
      antibody against the melanosome-specific protein
      gp100
      2. Phycoerythrin (PE)-conjugated antibody against
      the keratinocyte-specific marker cytokeratin.
      3. PE conjugated antibody against collagen 1, type
      1
      All are used for flowcytometry. Antibodies 1&2
      will be used together

      Read More
      Answer

      Thank you for contacting us.

      We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

      Click here (or use the following: https://www.abcam.com/PE-pan-Cytokeratin-antibody-C-11-ab52460.html).

      For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.

      Collagen I - ab23446
      EasyLink PE - ab102919 or ab102918

      For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.

      Click here (or use the following: https://www.abcam.com/Melanoma-gp100-antibody-OTI7E3-ab117853.html).

      Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG2b-heavy-chain-FITC-preadsorbed-ab98702.html).

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      We are currently using PE-C11 conjugate (ab 52460). Would it be possible to know F/P ratio of PE-C11 conjuagte

      Read More
      Answer

      Thank you for your enquiry and your interest in our products. I can confirm that the ratio is 1:1. I hope this information will be useful for you. Have a nice day,

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com