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  1. Link

    pe-pan-cytokeratin-antibody-c-11-ab52460.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (14)

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Abpromise

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Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

    Key features and details

    • PE Mouse monoclonal [C-11] to pan Cytokeratin
    • Suitable for: Flow Cyt
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-pan Cytokeratin antibody [C-11]
      See all pan Cytokeratin primary antibodies
    • Description

      PE Mouse monoclonal [C-11] to pan Cytokeratin
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Specificity

      ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with all species (recognized epitope conserved within all species)
    • Immunogen

      Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE.
    • Clonality

      Monoclonal
    • Clone number

      C-11
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class I
      • Cytokeratins

    Associated products

    • Alternative Versions

      • Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11] (ab277270)
      • Anti-pan Cytokeratin antibody [C-11] (ab7753)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

    Applications

    Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1-2µl for 106 cells.

    (in a 100µl cell suspension).

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Relevance

      Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
    • Cellular localization

      Cytoplasmic
    • Alternative names

      • pan ck antibody
      • pan-ck antibody
      • panck antibody

    Images

    • Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
      Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)

      Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

      Recommended conc. 0.5 μg/ml

      Overlay with Isotype mouse IgG1 control.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (14)

    Publishing research using ab52460? Please let us know so that we can cite the reference in this datasheet.

    ab52460 has been referenced in 14 publications.

    • Sun Y  et al. CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients. EBioMedicine 46:133-149 (2019). PubMed: 31375425
    • Karin O  et al. Senescent cell turnover slows with age providing an explanation for the Gompertz law. Nat Commun 10:5495 (2019). PubMed: 31792199
    • Singh M  et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res 79:2962-2977 (2019). PubMed: 30952634
    • Guo YX  et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
    • Lee H  et al. Functional Evidence of Pulmonary Extracellular Vesicles in Infectious and Noninfectious Lung Inflammation. J Immunol 201:1500-1509 (2018). PubMed: 29997122
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Flow Cytometry abreview for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (kidney, epithelial)
    Permeabilization
    Yes - Biolegend Intracellular Staining Permeabilization Wash Buffer
    Gating Strategy
    all live cells
    Specification
    kidney, epithelial
    Preparation
    Cell harvesting/tissue preparation method: primary cells passaged and trypisinzed from flask at p2
    Sample buffer: Biolegend Cell Staining Buffer (paraformaldehyde based)
    Fixation
    Biolegend Flurofix
    Read More

    Miss. Kara Shumansky

    Verified customer

    Submitted Mar 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (kidney, proximal tubule)
    Permeabilization
    Yes - 1% triton
    Specification
    kidney, proximal tubule
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Kara Shumansky

    Verified customer

    Submitted Feb 25 2019

    Question


    Request you to please let me know whether abcam
    can supply the following antibodies (reactivity:
    human)
    1. Fluorescein isothiocyanate (FITC)-conjugated
    antibody against the melanosome-specific protein
    gp100
    2. Phycoerythrin (PE)-conjugated antibody against
    the keratinocyte-specific marker cytokeratin.
    3. PE conjugated antibody against collagen 1, type
    1
    All are used for flowcytometry. Antibodies 1&2
    will be used together

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2012

    Answer

    Thank you for contacting us.

    We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=52460).

    For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.

    Collagen I - ab23446
    EasyLink PE - ab102919 or ab102918

    For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=117853).

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=98702).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2012

    Question

    We are currently using PE-C11 conjugate (ab 52460). Would it be possible to know F/P ratio of PE-C11 conjuagte

    Read More

    Abcam community

    Verified customer

    Asked on Jan 16 2009

    Answer

    Thank you for your enquiry and your interest in our products. I can confirm that the ratio is 1:1. I hope this information will be useful for you. Have a nice day,

    Read More

    Abcam Scientific Support

    Answered on Jan 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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