PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
![Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)](https://www.abcam.com/ps/products/52/ab52460/Images/ab52460-265341-anti-pan-cytokeratin-antibody-c-11-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Key features and details
- PE Mouse monoclonal [C-11] to pan Cytokeratin
- Suitable for: Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-pan Cytokeratin antibody [C-11]
See all pan Cytokeratin primary antibodies -
Description
PE Mouse monoclonal [C-11] to pan Cytokeratin -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with all species (recognized epitope conserved within all species) -
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
C-11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab52460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1-2µl for 106 cells. (in a 100µl cell suspension). ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
Cellular localization
Cytoplasmic -
Alternative names
- pan ck antibody
- pan-ck antibody
- panck antibody
Images
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Flow Cytometry - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 μg/ml
Overlay with Isotype mouse IgG1 control.
References (14)
ab52460 has been referenced in 14 publications.
- Sun Y et al. CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients. EBioMedicine 46:133-149 (2019). PubMed: 31375425
- Karin O et al. Senescent cell turnover slows with age providing an explanation for the Gompertz law. Nat Commun 10:5495 (2019). PubMed: 31792199
- Singh M et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res 79:2962-2977 (2019). PubMed: 30952634
- Guo YX et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
- Lee H et al. Functional Evidence of Pulmonary Extracellular Vesicles in Infectious and Noninfectious Lung Inflammation. J Immunol 201:1500-1509 (2018). PubMed: 29997122
