PE Anti-PD-L2 antibody [TY25] (ab93589)
![Flow Cytometry - PE Anti-PD-L2 antibody [TY25] (ab93589)](https://www.abcam.com/ps/products/93/ab93589/Images/ab93589-108625-anti-pd-l2-antibody-ty25-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-PD-L2 antibody [TY25] (ab93589)")
Key features and details
- PE Rat monoclonal [TY25] to PD-L2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-PD-L2 antibody [TY25]
See all PD-L2 primary antibodies -
Description
PE Rat monoclonal [TY25] to PD-L2 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse PD-L2. Mouse PD-L2 transfected cell line.
Database link: Q9BQ51 -
Positive control
- Mouse splenocyte suspensions and PD-L2 transfected cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TY25 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab93589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.06-0.5µg for 105-8 cells. |
Target
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. -
Tissue specificity
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted; Cell membrane and Endomembrane system. - Information by UniProt
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Database links
- Entrez Gene: 58205 Mouse
- SwissProt: Q9WUL5 Mouse
- Unigene: 116737 Mouse
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Alternative names
- B7 dendritic cell molecule antibody
- B7-DC antibody
- B7DC antibody
see all
Images
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Flow Cytometry - PE Anti-PD-L2 antibody [TY25] (ab93589)
Staining of non-transfected (left) and mouse PD-L2 transfected (right) cells with 0.25µg PE Rat IgG2a isotype control (open histogram) or 0.06µg ab93589 (colored histogram). Total viable cells were used for analysis.
References (4)
ab93589 has been referenced in 4 publications.
- Tanaka K et al. PDL1 is required for peripheral transplantation tolerance and protection from chronic allograft rejection. J Immunol 179:5204-10 (2007). PubMed: 17911605
- Kanai T et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol 171:4156-63 (2003). PubMed: 14530338
- Ansari MJ et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med 198:63-9 (2003). PubMed: 12847137
- Yamazaki T et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol 169:5538-45 (2002). PubMed: 12421930
