Recombinant PE Anti-Prohibitin antibody [EP2803Y] (ab210424)
![Flow Cytometry - PE Anti-Prohibitin antibody [EP2803Y] (ab210424)](https://www.abcam.com/ps/products/210/ab210424/Images/ab210424-260243-anti-prohibitin-antibody-ep2803y-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Prohibitin antibody [EP2803Y] (ab210424)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP2803Y] to Prohibitin
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Prohibitin antibody [EP2803Y]
See all Prohibitin primary antibodies -
Description
PE Rabbit monoclonal [EP2803Y] to Prohibitin -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Prohibitin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P35232 -
Positive control
- Flow Cyt: HeLa cells ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2803Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab210424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. | |
| ICC/IF | 1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
Target
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Function
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. -
Tissue specificity
Widely expressed in different tissues. -
Sequence similarities
Belongs to the prohibitin family. -
Developmental stage
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 5245 Human
- Entrez Gene: 18673 Mouse
- Entrez Gene: 25344 Rat
- Omim: 176705 Human
- SwissProt: P35232 Human
- SwissProt: P67778 Mouse
- SwissProt: P67779 Rat
- Unigene: 514303 Human
see all -
Alternative names
- Epididymis luminal protein 215 antibody
- Epididymis secretory sperm binding protein Li 54e antibody
- HEL 215 antibody
see all
Images
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Flow Cytometry - PE Anti-Prohibitin antibody [EP2803Y] (ab210424)
Overlay histogram showing HeLa cells stained with ab210424 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210424, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - PE Anti-Prohibitin antibody [EP2803Y] (ab210424)](https://www.abcam.com/ps/products/210/ab210424/Images/ab210424-259879-anti-prohibitin-antibody-ep2803y-phycoerythrin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - PE Anti-Prohibitin antibody [EP2803Y] (ab210424)ab210424 staining Prohibitin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210424 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
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![PE Anti-Prohibitin antibody [EP2803Y] (ab210424)](https://www.abcam.com/ps/products/210/ab210424/Images/ab210424-3-benefits-of-recombinant-antibodies.png)
PE Anti-Prohibitin antibody [EP2803Y] (ab210424)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab210424 has not yet been referenced specifically in any publications.
