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  1. Link

    pe-rabbit-igg-isotype-control-ab37407.pdf

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Isotype/Loading Controls Isotype Controls Rabbit
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PE-Rabbit IgG - Isotype Control (ab37407)

  • Datasheet
  • SDS
Q&A (4)References (2)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Overview

  • Product name

    PE-Rabbit IgG - Isotype Control
    See all Rabbit isotype controls
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Specificity

    This antibody has been selected to be an isotype control as it has no known specificity.
  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: 16% Sucrose, PBS

    Also contains stabilising agent.
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Rabbit
  • Alternative names

    • rabbit isotype control

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Our Abpromise guarantee covers the use of ab37407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.
ELISA Use at an assay dependent dilution.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab37407? Please let us know so that we can cite the reference in this datasheet.

    ab37407 has been referenced in 2 publications.

    • Bunkholt Elstrand M  et al. Mammalian target of rapamycin is a biomarker of poor survival in metastatic serous ovarian carcinoma. Hum Pathol 41:794-804 (2010). PubMed: 20153512
    • Dong HP  et al. Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry. Nat Protoc 3:955-64 (2008). PubMed: 18546596

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-4 of 4 Q&A

    Question



    Ein Kunde möchte die Konzentration wissen, wenn Sie Artikel Nr. ab37407 und ab72331 zusammen benutzt!



    Danke DIr

    Read More

    Abcam community

    Verified customer

    Asked on Mar 09 2012

    Answer

    Vielen Dank für Ihre Anfrage.
    Ich schlage vor die optimale Verdünnung für ab72331 zuerst zu testen und dann die Isotyp- Kontrolle (ab37407) in derselben Konzentration einzusetzen.
    Die Konzentration von ab37407 ist Lot abhängig. Liegt aber meistens in etwa bei 0.1mg/ml. Bitte teilen Sie mir das Lot mit und ich kann Ihnen eine genauere Auskunft geben.
    ab72331 hat eine Konzentration von 0.5mg/ml.
    Ich hoffe diese Auskunft ist hilfreich. Bitte zögern Sie nicht mich nochmals mit der Lot -nummer zu kontaktieren.

    Read More

    Abcam Scientific Support

    Answered on Mar 09 2012

    Question

    Dear Sir or Madam,
    Could you please tell me the concentration and volume of product
    ab37407, Rabbit IgG Phycoerythrin Isotype Control ?
    Thank you and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 18 2012

    Answer

    Thank you for your inquiry.
    We offer ab37407 as 100 tests. We recommend to use 10ul for one test (106 cells).
    I can confirm that this product has a volume of 1ml.
    The concentration of ab37407 is lot dependent. The lot that is in stock at the moment has a concentration of 0.01mg/mL.
    I hope this information is helpful. Please do not hesitate to contact me again with any further questions or the lot number that you received.

    Read More

    Abcam Scientific Support

    Answered on Jan 18 2012

    Question

    Please send the concentrations of ab37406, lot GR45440-2 ab37407, lot GR44588-1.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2011

    Answer

    Thank you for contacting us. The IgG concentrations are: ab37406, lot GR45440-2: 0.1 mg/ml ab37407, lot GR44588-1: 0.01 mg/ml I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 04 2011

    Question

    I am looking for an isotype control for Anti-Hsp90 beta antibody (Phycoerythrin) (ab115641). Is the following product of ab37407 the right one? https://www.abcam.com/Rabbit-IgG-Phycoerythrin-Isotype-Control-ab37407.html

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for your enquiry. The antibody ab37407 will be a suitable isotype control but please note that the stock concentration of the current batch of the control is 0.01 mg/ml, compared to 1.0 mg/ml for ab115641. To match the working concentrations of IgG you will need to dilute the HSP90 antibody stock solution 100-fold compared to the control. If you are working with live cells, consider that the amount of azide in each working solution will differ. Please let me know if you have any questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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