PE-Rabbit IgG - Isotype Control (ab37407)

Q&A (4)References (2)

Overview

  • Product name

    PE-Rabbit IgG - Isotype Control
    See all Rabbit isotype controls

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody has been selected to be an isotype control as it has no known specificity.

  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: 16% Sucrose, PBS

    Also contains stabilising agent.
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

  • Alternative names

    • rabbit isotype control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 10µl for 106 cells.
ELISA
Use at an assay dependent dilution.
Notes
Flow Cyt
Use 10µl for 106 cells.
ELISA
Use at an assay dependent dilution.

References (2)

Publishing research using ab37407? Please let us know so that we can cite the reference in this datasheet.

ab37407 has been referenced in 2 publications.

  • Bunkholt Elstrand M  et al. Mammalian target of rapamycin is a biomarker of poor survival in metastatic serous ovarian carcinoma. Hum Pathol 41:794-804 (2010). PubMed: 20153512
  • Dong HP  et al. Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry. Nat Protoc 3:955-64 (2008). PubMed: 18546596

Customer reviews and Q&As

Q&A
Submit a question

1-4 of 4 Q&A

Question



Ein Kunde möchte die Konzentration wissen, wenn Sie Artikel Nr. ab37407 und ab72331 zusammen benutzt!



Danke DIr

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Answer

Vielen Dank für Ihre Anfrage.
Ich schlage vor die optimale Verdünnung für ab72331 zuerst zu testen und dann die Isotyp- Kontrolle (ab37407) in derselben Konzentration einzusetzen.
Die Konzentration von ab37407 ist Lot abhängig. Liegt aber meistens in etwa bei 0.1mg/ml. Bitte teilen Sie mir das Lot mit und ich kann Ihnen eine genauere Auskunft geben.
ab72331 hat eine Konzentration von 0.5mg/ml.
Ich hoffe diese Auskunft ist hilfreich. Bitte zögern Sie nicht mich nochmals mit der Lot -nummer zu kontaktieren.

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Question

Dear Sir or Madam,
Could you please tell me the concentration and volume of product
ab37407, Rabbit IgG Phycoerythrin Isotype Control ?
Thank you and best regards,

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Answer

Thank you for your inquiry.
We offer ab37407 as 100 tests. We recommend to use 10ul for one test (106 cells).
I can confirm that this product has a volume of 1ml.
The concentration of ab37407 is lot dependent. The lot that is in stock at the moment has a concentration of 0.01mg/mL.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions or the lot number that you received.

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Question

Please send the concentrations of ab37406, lot GR45440-2 ab37407, lot GR44588-1.

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Answer

Thank you for contacting us. The IgG concentrations are: ab37406, lot GR45440-2: 0.1 mg/ml ab37407, lot GR44588-1: 0.01 mg/ml I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I am looking for an isotype control for Anti-Hsp90 beta antibody (Phycoerythrin) (ab115641). Is the following product of ab37407 the right one? https://www.abcam.com/Rabbit-IgG-Phycoerythrin-Isotype-Control-ab37407.html

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Answer

Thank you for your enquiry. The antibody ab37407 will be a suitable isotype control but please note that the stock concentration of the current batch of the control is 0.01 mg/ml, compared to 1.0 mg/ml for ab115641. To match the working concentrations of IgG you will need to dilute the HSP90 antibody stock solution 100-fold compared to the control. If you are working with live cells, consider that the amount of azide in each working solution will differ. Please let me know if you have any questions.

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