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  1. Link

    pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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RecombinantRabMAb

Recombinant PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

  • Datasheet
  • SDS
Submit a question References (2)

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Immunocytochemistry/ Immunofluorescence - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

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Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free FITC HRP PerCP Unconjugated

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Overview

  • Product name

    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
    See all IgG isotype controls
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.

  • General notes

    KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR25A
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Associated products

  • Alternative Versions

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)
    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
    • Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
    • Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
    • Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
    • PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)
    • FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)
    • APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)
  • Recombinant Protein

    • Recombinant Human IgG1 Fc Protein, His Tag (Active) (ab219660)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Flow Cyt
Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Images

  • Immunocytochemistry/ Immunofluorescence - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Immunocytochemistry/ Immunofluorescence - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 100% methanol (5 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes and blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and ab190573, Rabbit monoclonal to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive outcome under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10min).

  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Flow cytometry overlay histogram showing HeLa cells stained with ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209446) (1x106 in 100µL at 0.01µg/mL, 0.1µg/mL, 1µg/mL (1/50000, 1/5000, 1/500)) for 30 min at 22°C.

    Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Overlay histogram showing HeLa cells stained with ab208741 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208741, 1/500 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG monoclonal [EPR25A] (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

  • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab209478? Please let us know so that we can cite the reference in this datasheet.

ab209478 has been referenced in 2 publications.

  • Wu S  et al. The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization. Aging (Albany NY) 12:25845-25864 (2020). PubMed: 33234721
  • Zhu L  et al. Spermine on Endothelial Extracellular Vesicles Mediates Smoking-Induced Pulmonary Hypertension Partially Through Calcium-Sensing Receptor. Arterioscler Thromb Vasc Biol 39:482-495 (2019). PubMed: 30626206

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