RecombinantRabMAb

Recombinant PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Submit a question References (1)
Immunocytochemistry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Overview

  • Product name

    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
    See all IgG isotype controls

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Immunogen

    Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.

  • General notes

    KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR25A

  • Isotype

    IgG

  • Research areas

  • Cellular localization

    Secreted

  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Applications

Our Abpromise guarantee covers the use of ab209478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Flow Cyt Use at an assay dependent concentration.

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Images

  • Immunocytochemistry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Immunocytochemistry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/50 dilution (showing no signal) and ab195884, Rat monoclonal [YOL1/34] to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Flow cytometry overlay histogram showing HeLa cells stained with ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209446) (1x106 in 100µL at 0.01µg/mL, 0.1µg/mL, 1µg/mL (1/50000, 1/5000, 1/500)) for 30 min at 22°C.

    Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

    Overlay histogram showing HeLa cells stained with ab208741 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208741, 1/500 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG monoclonal [EPR25A] (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

  • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
    PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

References (1)

Publishing research using ab209478? Please let us know so that we can cite the reference in this datasheet.

ab209478 has been referenced in 1 publication.

  • Zhu L  et al. Spermine on Endothelial Extracellular Vesicles Mediates Smoking-Induced Pulmonary Hypertension Partially Through Calcium-Sensing Receptor. Arterioscler Thromb Vasc Biol 39:482-495 (2019). PubMed: 30626206

Customer reviews and Q&As

Q&A
Submit a question

There are currently no Questions for ab209478.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com