Recombinant PE Anti-RAGE antibody [EPR21171] (ab237363)
![Immunocytochemistry - PE Anti-RAGE antibody [EPR21171] (ab237363)](https://www.abcam.com/ps/products/237/ab237363/Images/ab237363-447549-AntiRAGE-antibody-EPR21171-PE-immunocytochemistry-transfected-hek293t.jpg "Immunocytochemistry - PE Anti-RAGE antibody [EPR21171] (ab237363)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR21171] to RAGE
- Suitable for: Flow Cyt, ICC
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-RAGE antibody [EPR21171]
See all RAGE primary antibodies -
Description
PE Rabbit monoclonal [EPR21171] to RAGE -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Immunogen
Recombinant fragment within Human RAGE aa 1-350. The exact sequence is proprietary.
Database link: Q15109 -
Positive control
- Flow Cyt: HEK-293T cells transfected with RAGE with myc tag. ICC: HEK-293T cells transfected with RAGE with myc tag.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21171 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab237363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. | |
| ICC | 1/50. |
Target
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Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
Images
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Immunocytochemistry - PE Anti-RAGE antibody [EPR21171] (ab237363)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells transfected with RAGE with myc tag labelling RAGE with ab237363 at 1/50 (10 µg/ml). The nuclear counterstain was DAPI (Blue).
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![Flow Cytometry - PE Anti-RAGE antibody [EPR21171] (ab237363)](https://www.abcam.com/ps/products/237/ab237363/Images/ab237363-447548-AntiRAGE-antibody-EPR21171-PE-flow-cytometry-transfected-hek293t.jpg)
Flow Cytometry - PE Anti-RAGE antibody [EPR21171] (ab237363)Flow cytometric analysis of 2% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with RAGE with myc tag cells labelling RAGE with ab237363 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).
Blocking with 10% Goat serum.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab237363 has not yet been referenced specifically in any publications.
