PE Anti-RANKL antibody [IK22/5] (ab93553)
![Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)](https://www.abcam.com/ps/products/93/ab93553/Images/RANKL-Primary-antibodies-ab93553-2.gif "Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)")
Key features and details
- PE Rat monoclonal [IK22/5] to RANKL
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
-
Product name
PE Anti-RANKL antibody [IK22/5]
See all RANKL primary antibodies -
Description
PE Rat monoclonal [IK22/5] to RANKL -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
NSO derived recombinant mouse RANKL.
-
Positive control
- 3-day ConA activated BALB/c splenocytes.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
IK22/5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
| Notes |
|---|
Application notes
Flow Cyt: Use at a concentration of 0.125 - 0.25 µg/test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
Tissue specificity
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
Involvement in disease
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localization
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 21943 Mouse
- SwissProt: O35235 Mouse
- Unigene: 249221 Mouse
-
Alternative names
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
Images
-
Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)ab93553 at 0.125µg dilution staining RANKL in 3-day unstimulated BALB/c splenocytes by Flow Cytometry (shown in blue).
-
![Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)](https://www.abcam.com/ps/products/93/ab93553/Images/RANKL-Primary-antibodies-ab93553-3.gif)
Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)ab93553 at 0.125µg dilution staining RANKL in 3-day ConA activated BALB/c splenocytes by Flow Cytometry (shown in blue).
Datasheets and documents
-
Datasheet download
References (0)
ab93553 has not yet been referenced specifically in any publications.
