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  1. Link

    pe-rankl-antibody-ik225-ab93553.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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PE Anti-RANKL antibody [IK22/5] (ab93553)

  • Datasheet
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Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
  • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)

Key features and details

  • PE Rat monoclonal [IK22/5] to RANKL
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

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Overview

  • Product name

    PE Anti-RANKL antibody [IK22/5]
    See all RANKL primary antibodies
  • Description

    PE Rat monoclonal [IK22/5] to RANKL
  • Host species

    Rat
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    NSO derived recombinant mouse RANKL.

  • Positive control

    • 3-day ConA activated BALB/c splenocytes.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IK22/5
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Biotin Anti-RANKL antibody [IK22/5] (ab95720)
  • Recombinant Protein

    • Recombinant mouse RANKL protein (Active) (ab129136)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Notes
Application notes
Flow Cyt: Use at a concentration of 0.125 - 0.25 µg/test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificity

    Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in disease

    Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localization

    Cytoplasm; Secreted and Cell membrane.
  • Target information above from: UniProt accession O14788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21943 Mouse
    • SwissProt: O35235 Mouse
    • Unigene: 249221 Mouse
    • Alternative names

      • CD254 antibody
      • hRANKL2 antibody
      • ODF antibody
      • OPGL antibody
      • OPTB2 antibody
      • Osteoclast differentiation factor antibody
      • Osteoprotegerin ligand antibody
      • Rank Ligand antibody
      • RANKL antibody
      • Receptor activator of nuclear factor kappa B ligand antibody
      • Receptor activator of nuclear factor kappa-B ligand antibody
      • sOdf antibody
      • TNF related activation induced cytokine antibody
      • TNF-related activation-induced cytokine antibody
      • TNF11_HUMAN antibody
      • TNFSF 11 antibody
      • Tnfsf11 antibody
      • TRANCE antibody
      • Tumor necrosis factor (ligand) superfamily member 11 antibody
      • Tumor necrosis factor ligand superfamily member 11 antibody
      • Tumor necrosis factor ligand superfamily member 11, soluble form antibody
      see all

    Images

    • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
      Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
      ab93553 at 0.125µg dilution staining RANKL in 3-day unstimulated BALB/c splenocytes by Flow Cytometry (shown in blue).
    • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
      Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
      ab93553 at 0.125µg dilution staining RANKL in 3-day ConA activated BALB/c splenocytes by Flow Cytometry (shown in blue).

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab93553? Please let us know so that we can cite the reference in this datasheet.

    ab93553 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RANKL antibody [IK22/5] (Phycoerythrin)

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    No
    Specification
    fibroblasts
    Blocking step
    Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    no fixation
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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