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    pe-rankl-antibody-ik225-ab93553.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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PE Anti-RANKL antibody [IK22/5] (ab93553)

  • Datasheet
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Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
  • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)

Key features and details

  • PE Rat monoclonal [IK22/5] to RANKL
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

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Overview

  • Product name

    PE Anti-RANKL antibody [IK22/5]
    See all RANKL primary antibodies
  • Description

    PE Rat monoclonal [IK22/5] to RANKL
  • Host species

    Rat
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    NSO derived recombinant mouse RANKL.

  • Positive control

    • 3-day ConA activated BALB/c splenocytes.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    IK22/5
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Biotin Anti-RANKL antibody [IK22/5] (ab95720)
  • Recombinant Protein

    • Recombinant mouse RANKL protein (Active) (ab129136)

Applications

Our Abpromise guarantee covers the use of ab93553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at a concentration of 0.125 - 0.25 µg/test.
    A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
    • Tissue specificity

      Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
    • Involvement in disease

      Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
    • Cellular localization

      Cytoplasm; Secreted and Cell membrane.
    • Target information above from: UniProt accession O14788 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 21943 Mouse
      • SwissProt: O35235 Mouse
      • Unigene: 249221 Mouse
      • Alternative names

        • CD254 antibody
        • hRANKL2 antibody
        • ODF antibody
        • OPGL antibody
        • OPTB2 antibody
        • Osteoclast differentiation factor antibody
        • Osteoprotegerin ligand antibody
        • Rank Ligand antibody
        • RANKL antibody
        • Receptor activator of nuclear factor kappa B ligand antibody
        • Receptor activator of nuclear factor kappa-B ligand antibody
        • sOdf antibody
        • TNF related activation induced cytokine antibody
        • TNF-related activation-induced cytokine antibody
        • TNF11_HUMAN antibody
        • TNFSF 11 antibody
        • Tnfsf11 antibody
        • TRANCE antibody
        • Tumor necrosis factor (ligand) superfamily member 11 antibody
        • Tumor necrosis factor ligand superfamily member 11 antibody
        • Tumor necrosis factor ligand superfamily member 11, soluble form antibody
        see all

      Images

      • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
        Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
        ab93553 at 0.125µg dilution staining RANKL in 3-day unstimulated BALB/c splenocytes by Flow Cytometry (shown in blue).
      • Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
        Flow Cytometry - PE Anti-RANKL antibody [IK22/5] (ab93553)
        ab93553 at 0.125µg dilution staining RANKL in 3-day ConA activated BALB/c splenocytes by Flow Cytometry (shown in blue).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab93553? Please let us know so that we can cite the reference in this datasheet.

      ab93553 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-RANKL antibody [IK22/5] (Phycoerythrin)

      Good
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (fibroblasts)
      Permeabilization
      No
      Specification
      fibroblasts
      Blocking step
      Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
      Fixative
      no fixation
      Read More

      Abcam user community

      Verified customer

      Submitted May 30 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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