PE Rat IgG2a, monoclonal [2A3]  - Isotype Control (ab253047)

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Overview

  • Product name

    PE Rat IgG2a, monoclonal [2A3]  - Isotype Control
    See all IgG2a isotype controls

  • Description

    PE Rat IgG2a, monoclonal [2A3] - Isotype Control

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    ab253047 binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    Chemical/ Small Molecule. TNP hapten.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    2A3

  • Isotype

    IgG2a

  • Research areas

  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.

  • Cellular localization

    Secreted

Applications

Our Abpromise guarantee covers the use of ab253047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

This antibody preparation has been quality-tested for flow cytometry using an appropriate cell type. The isotype control should be used at the same concentration as the primary antibody for which it is a control.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab253047? Please let us know so that we can cite the reference in this datasheet.

ab253047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
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