Key features and details
- PE Rabbit monoclonal [EPR17792] to Renilla Luciferase
- Suitable for: Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product namePE Anti-Renilla Luciferase antibody [EPR17792]
See all Renilla Luciferase primary antibodies
DescriptionPE Rabbit monoclonal [EPR17792] to Renilla Luciferase
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Predicted to work with: Other speciesDoes not react with: Mouse, Rat, Human
Synthetic peptide within Renilla Luciferase aa 150-250. The exact sequence is proprietary.
Database link: P27652
- Flow Cyt: 293T cells transfected with RFP tagged Renilla Luciferase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab225340 in the following tested applications.
The cellular localisation of this product has been verified in ICC/IF.
RelevanceThe Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
- SwissProt: P27652 Other species
- Renilla luciferin 2 monooxygenase antibody
- Renilla type luciferase antibody
Overlay histogram showing 293T transfected with RFP tagged Renilla Luciferase stained with ab225340 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225340, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW blue solid-state laser (488nm) and 585/42 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225340 has not yet been referenced specifically in any publications.