RecombinantRabMAb

Recombinant PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)

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Flow Cytometry - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
  • Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
  • PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP786Y] to ROCK2 + ROCK1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • Description

    PE Rabbit monoclonal [EP786Y] to ROCK2 + ROCK1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ROCK1 aa 1100-1200 (C terminal). The exact sequence is proprietary.
    Database link: Q13464

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab224972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

Target

Images

  • Flow Cytometry - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
    Flow Cytometry - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)

    Overlay histogram showing HeLa cells stained with ab224972 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224972, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
    Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
    All lanes : Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution

    All lanes : Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab45171, the same antibody clone in a different buffer formulation.

    Loading control: Anti-6X His tag® antibody [EPR20547] (ab213204)

    Blocking buffer and concentration: 5% NFDM/TBST

    Exposure Times:

    Lane 1: 3 seconds

    Lane 2: 7 seconds

  • PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
    PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab224972? Please let us know so that we can cite the reference in this datasheet.

ab224972 has not yet been referenced specifically in any publications.

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