PE Anti-SIGLEC5 antibody [1A5] (ab240998)

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Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)

    Key features and details

    • PE Mouse monoclonal [1A5] to SIGLEC5
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Unconjugated

    Overview

    • Product name

      PE Anti-SIGLEC5 antibody [1A5]
      See all SIGLEC5 primary antibodies

    • Description

      PE Mouse monoclonal [1A5] to SIGLEC5

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant fragment (Fc-tag) corresponding to Human SIGLEC5 (extracellular). Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
      Database link: O15389

    • Positive control

      • Flow Cyt: Human peripheral blood cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    • Clonality

      Monoclonal

    • Clone number

      1A5

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab240998 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    (or 100 μl of whole blood).
    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    (or 100 μl of whole blood).

    Target

    • Function

      Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

    • Tissue specificity

      Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD170 antibody
        • CD170 antigen antibody
        • CD33 antigen-like 2 antibody
        • CD33L2 antibody
        • mSiglec-F antibody
        • OB-binding protein 2 antibody
        • OB-BP2 antibody
        • OBBP2 antibody
        • Obesity-binding protein 2 antibody
        • Sialic acid binding Ig-like lectin 5 antibody
        • Sialic acid binding immunoglobulin-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin F antibody
        • SIGL5_HUMAN antibody
        • Siglec-5 antibody
        • Siglec-F variant antibody
        • Siglec5 antibody
        • Siglecf antibody
        see all

      Images

      • Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)
        Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)

        Flow cytometric analysis of human peripheral blood cells labeling SIGLEC5 with ab240998. Surface staining.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab240998? Please let us know so that we can cite the reference in this datasheet.

      ab240998 has not yet been referenced specifically in any publications.

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