PE Anti-SIGLEC5 antibody [1A5] (ab240998)
![Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)](https://www.abcam.com/ps/products/240/ab240998/Images/ab240998-319771-anti-siglec5-antibody-1a5-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)")
Key features and details
- PE Mouse monoclonal [1A5] to SIGLEC5
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-SIGLEC5 antibody [1A5]
See all SIGLEC5 primary antibodies -
Description
PE Mouse monoclonal [1A5] to SIGLEC5 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein (Fc-tag) corresponding to Human SIGLEC5 (extracellular).
Database link: O15389 -
Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
1A5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. (or 100 μl of whole blood). |
Target
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Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. -
Tissue specificity
Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8778 Human
- Omim: 604200 Human
- SwissProt: O15389 Human
- Unigene: 310333 Human
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Alternative names
- CD170 antibody
- CD170 antigen antibody
- CD33 antigen-like 2 antibody
see all
Images
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Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)
Flow cytometric analysis of human peripheral blood cells labeling SIGLEC5 with ab240998. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240998 has not yet been referenced specifically in any publications.
