PE Anti-SIGLEC5 antibody [1A5] (ab240998)
![Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)](https://www.abcam.com/ps/products/240/ab240998/Images/ab240998-319771-anti-siglec5-antibody-1a5-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)")
Key features and details
- PE Mouse monoclonal [1A5] to SIGLEC5
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-SIGLEC5 antibody [1A5]
See all SIGLEC5 primary antibodies -
Description
PE Mouse monoclonal [1A5] to SIGLEC5 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Fc-tag) corresponding to Human SIGLEC5 (extracellular). Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
Database link: O15389 -
Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
1A5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
(or 100 μl of whole blood). |
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. (or 100 μl of whole blood). |
Target
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Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. -
Tissue specificity
Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8778 Human
- Omim: 604200 Human
- SwissProt: O15389 Human
- Unigene: 310333 Human
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Alternative names
- CD170 antibody
- CD170 antigen antibody
- CD33 antigen-like 2 antibody
see all
Images
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Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)
Flow cytometric analysis of human peripheral blood cells labeling SIGLEC5 with ab240998. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab240998 has not yet been referenced specifically in any publications.
