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    pe-siglec5-antibody-1a5-ab240998.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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PE Anti-SIGLEC5 antibody [1A5] (ab240998)

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Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)

    Key features and details

    • PE Mouse monoclonal [1A5] to SIGLEC5
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-SIGLEC5 antibody [1A5]
      See all SIGLEC5 primary antibodies
    • Description

      PE Mouse monoclonal [1A5] to SIGLEC5
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Fusion protein (Fc-tag) corresponding to Human SIGLEC5 (extracellular).
      Database link: O15389

    • Positive control

      • Flow Cyt: Human peripheral blood cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
    • Clonality

      Monoclonal
    • Clone number

      1A5
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion

    Associated products

    • Alternative Versions

      • Anti-SIGLEC5 antibody [1A5] (ab97426)
    • Recombinant Protein

      • Recombinant Human SIGLEC5 protein (Fc Chimera) (ab221413)

    Applications

    Our Abpromise guarantee covers the use of ab240998 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    (or 100 μl of whole blood).

    Target

    • Function

      Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
    • Tissue specificity

      Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession O15389 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8778 Human
      • Omim: 604200 Human
      • SwissProt: O15389 Human
      • Unigene: 310333 Human
      • Alternative names

        • CD170 antibody
        • CD170 antigen antibody
        • CD33 antigen-like 2 antibody
        • CD33L2 antibody
        • mSiglec-F antibody
        • OB-binding protein 2 antibody
        • OB-BP2 antibody
        • OBBP2 antibody
        • Obesity-binding protein 2 antibody
        • Sialic acid binding Ig-like lectin 5 antibody
        • Sialic acid binding immunoglobulin-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin 5 antibody
        • Sialic acid-binding Ig-like lectin F antibody
        • SIGL5_HUMAN antibody
        • Siglec-5 antibody
        • Siglec-F variant antibody
        • Siglec5 antibody
        • Siglecf antibody
        see all

      Images

      • Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)
        Flow Cytometry - PE Anti-SIGLEC5 antibody [1A5] (ab240998)

        Flow cytometric analysis of human peripheral blood cells labeling SIGLEC5 with ab240998. Surface staining.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab240998? Please let us know so that we can cite the reference in this datasheet.

      ab240998 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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