PE Anti-SIRPB1 antibody [B4B6] (ab253258)
![Flow Cytometry - PE Anti-SIRPB1 antibody [B4B6] (ab253258)](https://www.abcam.com/ps/products/253/ab253258/Images/ab253258-343915-AntiSIRP-beta-1-CD172b-antibody-B4B6-Phycoerythrin-flow-cytometry-human-peripheral-blood-cells.jpg "Flow Cytometry - PE Anti-SIRPB1 antibody [B4B6] (ab253258)")
Key features and details
- PE Mouse monoclonal [B4B6] to SIRPB1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-SIRPB1 antibody [B4B6]
See all SIRPB1 primary antibodies -
Description
PE Mouse monoclonal [B4B6] to SIRPB1 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to SIRP beta 1/CD172b. NIH/3T3 cells transfected with human CD172b.
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Positive control
- Flow cyt: Human peripheral blood cells.
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General notes
This product was previously labelled as SIRP beta 1/CD172b
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
B4B6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. Or 100 µl of whole blood. |
Target
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Relevance
The protein encoded by the SIRPß1 gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins which are known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPß1 was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Database links
- Entrez Gene: 10326 Human
- Omim: 603889 Human
- SwissProt: O00241 Human
- SwissProt: Q5TFQ8 Human
- Unigene: 664861 Human
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Alternative names
- CD172 antigen like family member B antibody
- CD172b antibody
- CD172b antigen antibody
see all
Images
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Flow Cytometry - PE Anti-SIRPB1 antibody [B4B6] (ab253258)
Flow cytometric analysis of human peripheral blood labeling SIRP beta 1/CD172b with ab253258. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253258 has not yet been referenced specifically in any publications.
