RecombinantRabMAb

Recombinant PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)

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Immunocytochemistry/ Immunofluorescence - PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
  • PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418)
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-SRC Family (phospho Y418) antibody [EP503Y]
    See all SRC Family primary antibodies

  • Description

    PE Rabbit monoclonal [EP503Y] to SRC Family (phospho Y418)

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    ab208828 recognises Lyn phosphorylation on Tyrosine 396. BLAST analysis show 100% homology with Hck (pY410) and Lck (pY393), and 92% homology with Src (pY418), Fyn (pY419) and Yes (pY425) Therefore, the antibody may cross-react with these proteins.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SRC Family aa 350-450 (phospho Y418). The exact sequence is proprietary.
    Database link: P07948
    (Peptide available as ab202645)

  • Positive control

    • ICC/IF: pervanadate-treated HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP503Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab208828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in pervanadate-treated HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Relevance

    SRC Family Negative Regulatory Phosphorylation Site belongs to the protein kinase superfamily, Tyr protein kinase family, SRC subfamily. It contains one protein kinase domain, one SH2 domain and one SH3 domain. It may serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst and may also contribute to neutrophil migration and may regulate the degranulation process of neutrophils. SRC Family Negative Regulatory Phosphorylation Site is expressed predominantly in cells of the myeloid and B lymphoid lineages. There are two named isoforms.

  • Cellular localization

    Isoform 1: Membrane; Lipid anchor. Isoform 2: Membrane; Lipid anchor. Cytoplasm.

  • Database links

    see all

  • Alternative names

    • ASV antibody
    • Protooncogene SRC, Rous sarcoma antibody
    • SRC antibody
    • SRC1 antibody

Images

  • Immunocytochemistry/ Immunofluorescence - PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
    Immunocytochemistry/ Immunofluorescence - PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)

    ab208828 staining SRC Family (phospho Y418) in HeLa cells +/- pervanadate (1mM, 15min), then treated with LP (31 degrees C, 2 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

    The cells were then incubated overnight at +4°C with ab208828 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)
    PE Anti-SRC Family (phospho Y418) antibody [EP503Y] (ab208828)

References (0)

Publishing research using ab208828? Please let us know so that we can cite the reference in this datasheet.

ab208828 has not yet been referenced specifically in any publications.

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